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EC number: 222-860-8 | CAS number: 3637-26-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-04-25 - 2016-06-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- OECD Guidelines for the Testing of Chemicals Part 301 F, adopted 17. Jul. 1992 „Manometric Respirometry Test“
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- Council Regulation (EC) No. 440/2008, Guideline C.4-D, adopted 30. May 2008 “Manometric Respirometry Test”
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- by the Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Kaiser-Friedrich-Straße 7, 55116 Mainz
Test material
- Reference substance name:
- Dimethyl[2-[(2-methyl-1-oxoallyl)oxy]ethyl](3-sulphopropyl)ammonium hydroxide
- EC Number:
- 222-860-8
- EC Name:
- Dimethyl[2-[(2-methyl-1-oxoallyl)oxy]ethyl](3-sulphopropyl)ammonium hydroxide
- Cas Number:
- 3637-26-1
- Molecular formula:
- C11H21NO5S
- IUPAC Name:
- dimethyl[2-[(2-methyl-1-oxoallyl)oxy]ethyl](3-sulphopropyl)ammonium hydroxide
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test item was stored in a closed vessel at 17.0 – 23.3 °C, away from humidity.
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from a biologic sewage treatment plant was used. The chosen plant is treating mostly household sewage.
The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf.
- Preparation of inoculum for exposure: The sludge was filtrated, washed with tap water (2x), then washed with and re-suspended in test medium. It was then aerated.
- Concentration of sludge: The dry matter was determined as 4960 mg suspended solids/L. - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 53 mg/L
- Based on:
- test mat.
- Initial conc.:
- 100 mg/L
- Based on:
- ThOD
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Stock solutions
a) 8.5 g KH2PO4, 21.75 g K2HPO4, 33.4 g Na2HPO4 x 2 H2O, 0.5 g NH4Cl and 1000 mL H2O demin. ad
b) 36.4 g CaCl2 x 2H2O and 1000 mL H2O demin. ad
c) 22.5 g MgSO4 x 7H2O and 1000 mL H2O demin. ad
d) 0.25 g FeCl3 x 6H2O, 0.4 g Na2EDTA x 2H2O and 1000 mL H2O demin. ad
Test Medium
10 mL solution a
1 mL solution b
1 mL solution c
1 mL solution d
1000 mL H2O demin. ad
- Test temperature: 20.5 – 23.7 °C
- pH adjusted: yes, the pH was measured with 7.6, the test abiotic test vessel was adjusted to 7.3.
- Aeration of dilution water: not stated
- Suspended solids concentration: 25 mg/l
TEST SYSTEM
- Culturing apparatus: 500 mL-flasks were used as test vessels.
All glassware was cleaned with the laboratory cleaning agent and then rinsed with tap water (thrice), diluted HCL (once), tap water (thrice) and deionised water (thrice).
- Number of culture flasks/concentration:
Number of controls: 3 (medium and inoculum)
Number of positive control vessels: 3 (sodium-benzoate, medium and inoculum)
Number of test vessels: 3 (test item, medium and inoculum)
Abiotic control: 1 (test item and 10 mL 1% HgCl2 solution/L)
Toxicity control: 1 (100 mg ThOD each of test item and positive control)
- Measuring equipment: The vessels were connected to the OxiTop® measuring heads and they were started via the OxiTop® Controllers.
- Other:
Volume of test vessels: 500 mL
Volume test solution: 250 mL (toxicity control flask 125 mL)
CONTROL AND BLANK SYSTEM
- Inoculum blank: 3 (medium and inoculum)
- Abiotic sterile control: 1 (test item and 10 mL 1% HgCl2 solution/L)
- Toxicity control: 1 (100 mg ThOD each of test item and positive control)
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Preliminary study:
- No information about a preliminary study are available.
- Test performance:
- No unusual observations during test period observed.
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 8
- Sampling time:
- 28 d
- Remarks on result:
- other:
- Remarks:
- The 10-day-window was not detected.
BOD5 / COD results
- Results with reference substance:
- Degradation after 3 days > 60% (validity criterion fulfilled)
Positive control 1: 88.1 % degradation after 28 days
Positive control 2: 84.5 % degradation after 28 days
Positive control : 79.1 % degradation after 28 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The study was performed according to OECD TG301F and EU Method C.4-D (GLP) without deviations and therefore considered to be of the highest quality (reliability Klimisch 1). The validity criteria of the test system are fulfilled. Within 28 days, a degradation of 8% was determined for the test substance; the 10 day window criterion was not passed. Therefore, the test substance is considered to be not readily biodegradable.
- Executive summary:
The study was conducted following OECD 301F / EU C.4-D under GLP on N,N-Dimethyl-N-(2-methacryloyloxyethyl)-N-(3-sulfopropyl)-ammonium betaine; (SPE)
Two experiments were performed. In the first experiment, the measuring heads showed leakages and therefore inconsistent data. Therefore, the experiment was repeated under the same conditions. The results of the first experiment are not stated in this report, but will be kept together with the other raw data in the GLP archive of the test facility.
The test item N,N-Dimethyl-N-(2-methacryloyloxyethyl)-N-(3-sulfopropyl)-ammonium betaine; (SPE) was tested using a concentration of 53 mg test item/L (equivalent to a theoretical oxygen demand of 100.15 mg O2/L). Activated sludge was used as inoculum. The test was left running for 28 days.
All validity criteria were met. The positive control reached the pass level of 60% on day 3 (criterion:<14).
The following data could be determined for the test item N,N-Dimethyl-N-(2-methacryloyloxyethyl)-N-(3-sulfopropyl)-ammonium betaine; (SPE):
Lag phase: not detected
10-day-window:not detected
degradation at the end of 10-day-window: none
degradation at the end of the test: 8 %
Therefore, regardless of the 10-day-window, N,N-Dimethyl-N-(2-methacryloyloxyethyl)-N-(3-sulfopropyl)-ammonium betaine; (SPE) is classified as not readily biodegradable following OECD 301F/EU C.4-D.
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