Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 229-904-5 | CAS number: 6829-22-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015-07-06 till 2015-09-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline study without restrictions.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.20 (Daphnia magna Reproduction Test)
- Version / remarks:
- (2008)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Version / remarks:
- (2012)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentration: 10 mg/L 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one plus control
- Sampling schedule: Control of on day 0, 9 and 19 in the freshly prepared media; on day 2, 12 and 21 in the old media and test concentration of 10 mg/L on day 0, 9 and 19 in the freshly prepared media; on day 2, 12 and 21 in the old media
- Sample storage: Routinely, the samples were analysed immediately. Only in exceptional cases, they were stored overnight deep frozen and protected from light. - Vehicle:
- no
- Details on test solutions:
- Pre-treatment of 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one
A direct weighing was prepared to produce the only test concentration.
20 mg of 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one were added to 2 litres of dilution water (day 0 – 8 and 16) and 10 mg of 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one were added to 1 litre of dilution water (day 9 – 15 ), treated for 1 hour in an ultrasonic bath and stirred for 24 h on a magnetic stirrer. Finally undissolved particles of 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one were removed by filtration using a folded filter with a pore size of 7-12 μm. The pH was measured and ranged between pH 7.7 and 7.8. 100 mL of the prepared solution and one neonate was added to each replicate. For each test item concentration and the control 10 replicates were prepared. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Name : Daphnia magna STRAUS, parthenogenetic females
- Source : Strain of Bundesgesundheitsamt Berlin
- Maintenance and Acclimatisation : A population of parthenogenetic females of synchronized age structure has been maintained for more than 20 years in the test facility under constant temperature conditions (20 +/- 1 °C) at a 16 : 8 hour light-dark photoperiod (light intensity: < 20 μE x m-2 x s-1). The culture water (so-called 'M4 medium') was partly renewed once a week. The Daphnia were exclusively fed unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period were recorded daily in a semi-quantitative way. The neonates were separated from their parent Daphnia by filtration prior to the acute test. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 21 d
- Hardness:
- 13.4 °dH to 15.0 °dH (= 239 - 268 mg/L CaCO3) measured at each test vessel at the beginning of the test and at 2, 9, 12, 19 and 21 days
- Test temperature:
- 20.1 - 20.9 °C measured at each test vessel at the beginning of the test and at 2, 9, 12, 19 and 21 days
- pH:
- 7.8 - 8.1 measured at each test vessel at the beginning of the test and at 2, 9, 12, 19 and 21 days
- Dissolved oxygen:
- 8.7 - 9.1 mg O2/L with 102 - 105 % saturation measured at each test vessel at the beginning of the test and at 2, 9, 12, 19 and 21 days
- Nominal and measured concentrations:
- - Concentration: 10 mg/L 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one plus control
The results are expressed in terms of time-weighted mean measured concentrations. Effective concentrations ranged from 5.3 % to 7.3 % of the nominal values in the freshly prepared media and from 3.8 % to 5.5 % in the media after 48 hours, or 72 hours of exposure. - Details on test conditions:
- Exposure conditions
- Test vessels: 150 mL glass beakers covered with watch glasses, holding 1 neonate in 100 mL test medium
- Experimental design: 1 test concentration plus 1 control; 1 neonate per vessel, 10 replicates per concentration/control; Semi static system (renewal of the test media on Monday, Wednesday and Friday)
- Method of initiation- : neonates were placed in prepared media
Photoperiod: 16 h light : 8 h dark
- Light intensity: not exceeding 20 μE x m-2 x s-1
- Temperature of incubation unit: 19.5 to 20.5 °C
- Aeration: none
- Test item concentration: 10 mg/L
- Food source and feeding rate: Desmodesmus subspicatus; day 0-7: 0.1 mg C / Daphnia / day and day 8-21: 0.2 mg C / Daphnia / day
- Duration of exposure: 21 days
-Criteria of effects : The criterion of adverse effects used in this study was the item-induced inhibitory effect on the parthenogenetic reproduction rate and the mobility behaviour/mortality rate of parent Daphnia, all effects being assessed at least three times a week. - Reference substance (positive control):
- no
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.566 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 0.566 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.566 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 0.566 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- 1st brood on day: 8
- Validity criteria fulfilled:
- yes
- Remarks:
- (- The mortality rate in the controls did not exceed 20 % by the end of the test. - The dissolved oxygen concentration remained above 3 mg/L throughout the exposure period.)
- Conclusions:
- Daphnia magna was exposed under semi-static conditions for 21 days to one concentration (10 mg/L) of 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one. No toxic effects against daphnia were observed at a test concentration of nominally 10 mg/L, corresponding to a time-weighted mean measured concentration of 0.566 mg/L, which reflects the maximum water solubility under exposure conditions.
- Executive summary:
A study was performed to assess the effects of 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one on the reproductive output of parthenogenetic Daphnia magna to the test solution of one concentration (10 mg/L) and blank control solution under semi static conditions.
The study was conducted in accordance with Council Regulation (EC) No 440/2008, Method C.20 'Daphnia magna Reproduction Test'' (2008), which equals in all relevant parts to the OECD Test guideline 211 (Daphnia magna Reproduction Test; 2012).
Base on different biological effects observed and endpoints assessed, the following results were obtained at the end of the 21 day exposure of the Daphnia reproduction test:
21d-NOEC (reproduction): ≥ 0.566 mg/L
21d-LOEC (reproduction): > 0.566 mg/L
21d-NOEC (mortality): ≥ 0.566 mg/L
21d-LOEC (mortality): > 0.566 mg/L
1st brood on day : 8
No toxic effects against daphnia were observed at a test concentration of nominally 10 mg/L, corresponding to a time-weighted mean measured concentration of 0.566 mg/L, which reflects the maximum water solubility under exposure conditions.
The results are expressed in terms of time-weighted mean measured concentrations. Effective concentrations ranged from 5.3 % to 7.3 % of the nominal values in the freshly prepared media and from 3.8 % to 5.5 % in the media after 48 hours, or 72 hours of exposure.
Reference
Description of key information
Daphnia magna was exposed under semi-static conditions for 21 days to one
concentration (10 mg/L) of 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one. No toxic effects against daphnia were observed at a test concentration of nominally 10 mg/L, corresponding to a time-weighted mean measured concentration of 0.566 mg/L, which reflects the maximum water solubility under exposure conditions.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.057 mg/L
Additional information
The key value should read as >= 0.566 mg/L as there was no effect up to the maximum water solubility of the substance and, thus, the substance has no chronic effects on daphnids.
Discussion
A study was performed to assess the effects of 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one on the reproductive output of parthenogenetic Daphnia magna to the test solution of one concentration (10 mg/L) and blank control solution under semi static conditions.
The study was conducted in accordance with Council Regulation (EC) No 440/2008, Method C.20 'Daphnia magna Reproduction Test'' (2008), which equals in all relevant parts to the OECD Test guideline 211 (Daphnia magna Reproduction Test; 2012).
Base on different biological effects observed and endpoints assessed, the following results were obtained at the end of the 21 day exposure of the Daphnia reproduction test:
21d-NOEC (reproduction): ≥ 0.566 mg/L
21d-LOEC (reproduction): > 0.566 mg/L
21d-NOEC (mortality): ≥ 0.566 mg/L
21d-LOEC (mortality): > 0.566 mg/L
1stbrood on day : 8
No toxic effects against daphnia were observed at a test concentration of nominally 10 mg/L, corresponding to a time-weighted mean measured concentration of 0.566 mg/L, which reflects the maximum water solubility under exposure conditions.
The results are expressed in terms of time-weighted mean measured concentrations. Effective concentrations ranged from 5.3 % to 7.3 % of the nominal values in the freshly prepared media and from 3.8 % to 5.5 % in the media after 48 hours, or 72 hours of exposure.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.