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EC number: 289-068-2 | CAS number: 85959-73-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with analytical monitoring. Concentration-effect relationship ambiguous but acceptable.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- The temperature ranged from 23.8 to 27.2 °C, which is slightly out of the required range of 24± 2 °C.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- yes
- Details on test solutions:
- To prepare the standard solutions, a stock solution (test item dissolved in ultra-pure water:acetonitrile (1:1, v:v)) was diluted with medium:methanol (8:2; v:v) containing 10 mM ammonium acetate. Test samples were diluted to obtain a final sample composition of sample:methanol (8:2, v:v) containing 10mM ammonium acetate before HPLC analysis.
- Test organisms (species):
- Lemna minor
- Details on test organisms:
- Test organism: Exponential growing plant monoculture of Lemna minor (Umweltbundesamt, FGIII 2.5, Überwachungsverfahren Abwasserentsorgung, Schichauweg 58, D-12307 Berlin)
Culture: All-glass vessel containing sterile Swedish standard-Medium (SIS Medium) and the exponentially growing plant monoculture.
Cultivation: At least seven days before testing, sufficient colonies were transferred aseptically into fresh sterile medium and cultured for 7-10 days under the conditions of the test.
Illumination: Continuous (6500–10000 lux) from Osram Fluora L18W77 (Osram AG, Winterthur, Switzerland) and CH Lighting F18T8/6500K EUP (CH Lighting CO., Ltd., China)
Temperature 24 ± 2 °C - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Test temperature:
- 24 ± 2 °C
- pH:
- 6.5 at the beginning of the test
- Dissolved oxygen:
- Determined and reported.
- Nominal and measured concentrations:
- Prior to the definitive test a non-GLP range finding test with the nominal concentrations of 1, 10 and 100 mg/l of active ingredient was performed.
Based on the results of the non-GLP range finding test, the test concentrations for the definitive test were 326, 102, 31.8, 9.95 and 3.11 mg/l nominal concentration of the test item, corresponding to 250, 78.1, 24.4, 7.63 and 2.38 mg/l of the active ingredient. - Details on test conditions:
- Test vessel: 400 ml beakers, all-glass, with 200 ml of test medium. The beakers were covered with black paper up until the 200 ml mark to ensure that illumination comes only from above and not from the sides.
Test medium: Swedish standard-Medium (SIS Medium), prepared with ultra-pure water
Pre-culture: Exponentially growing plant monoculture of Lemna minor (the culture was visibly free from contamination by other organisms such as algae and protozoa; only healthy Lemna minor plants with comprising 2-4 fronds were used).
Illumination Intensity: continuous (6500–10000 lux) from Osram Fluora L18W77 (Osram AG, Winterthur, Switzerland) and CH Lighting F18T8/6500K EUP (CH Lighting CO., Ltd., China) Homogeneity: ±15%
Incubation Beakers were incubated on a black non-reflecting surface. - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
- Duration:
- 7 d
- Dose descriptor:
- EC10
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: nominal value analytically verified
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 196 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: nominal value analytically verified
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.38 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 7 d
- Dose descriptor:
- EC10
- Effect conc.:
- 3.36 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: nominal value analytically verified, 95%CL: n.d. - 8.67 mg/L)
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 88.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: nominal value analytically verified
- Details on results:
- EFFECT ON FROND NUMBERS:
With respect to growth rate inhibition, the following effects as compared to the untreated controls were observed: 55% at 250 mg/l, 32% at 78.1 mg/l and 14% at 24.4 mg/l. No significant effects were observed at the 7.63 and 2.38 mg/l concentrations. Based on these effects and the measured concentrations of the active ingredient, the median effect concentration with respect to the frond number’s growth rate (frond number ErC50) of the tested substance to Lemna minor was calculated to be 196 mg/l (95% confidence limits: 153–269 mg/l).
The ErC10 was 13.0 (6.56–20.4) mg/l; while the NOErC was determined to be 6.38 mg/l.
EFFECT ON DRY WEIGHT:
With respect to growth rate inhibition, the following effects as compared to the untreated controls were observed: 75% at 250 mg/l, 40% at 78.1 mg/l, 27% at 24.4 mg/l, 17% at 7.63 mg/l and 5% at 2.38 mg/l.
Based on these effects and the measured concentrations of the active ingredient, the median effect concentration with respect to the dry weight’s growth rate (dry weight r ErC50) of the tested substance to Lemna minor was calculated to be 88.4 mg/l (95% confidence limits: 35.1–251 mg/l). The ErC10 was 3.36 (0.012–13.3) mg/l. Due to the variation among replicates within the 3 lower concentrations, the NOErC was declared as not assessable. - Results with reference substance (positive control):
- -
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 = 196 mg/l
NOEC = 6.38 mg/l - Executive summary:
The inhibitory effects of Acid Yellow 194 to the duckweed Lemna minor were investigated over a period of 7 days, based on the frond number and biomass (dry weight), following the guideline OECD 221.
Based on the test results, the EC50 value was set at 196 mg/l.
Reference
Description of key information
The inhibitory effects of Acid Yellow to the duckweed Lemna minor were investigated over a period of 7 days, based on the frond number and biomass (dry weight), following the guideline OECD 221.
Based on the test results, the EC50 value was set at 196 mg/l and the NOEC value was set to 6.38 mg/l.
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 88.4 mg/L
- EC10 or NOEC for freshwater plants:
- 3.36 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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