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EC number: 214-122-9 | CAS number: 1087-21-4
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 April 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- not specified
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Diallyl isophthalate
- EC Number:
- 214-122-9
- EC Name:
- Diallyl isophthalate
- Cas Number:
- 1087-21-4
- Molecular formula:
- C14H14O4
- IUPAC Name:
- 1,3-bis(prop-2-en-1-yl) benzene-1,3-dicarboxylate
- Test material form:
- liquid
- Details on test material:
- - Name of test material (as cited in study report): Diallyl isophthalate
- Substance type: organic
- Physical state: liquid
- Analytical purity: 99.8%
- Purity test date:
- Lot/batch No.:
- Expiration date of the lot/batch:
- Stability under test conditions:
- Storage condition of test material:
- Other:
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9
- Test concentrations with justification for top dose:
- Without S9 mix
0, 2.29, 6.86, 20.6, 61.7, 185, 556, 1667 and 5000 µg/plate (all strains: dose determination test)
0, 4.88, 9.77, 19.5, 39.1, 78.1, 156 and 313 µg/plate (TA100: Main test)
0, 1.22, 2.44, 4.88, 9.77, 19.5, 39.1 and 78.1 µg/plate (TA1535: Main test)
0, 39.1, 78.1, 156, 313, 625, 1250, 2500 and 5000 µg/plate (WP2urvA/pKM101: Main test)
0, 2.44, 4.88, 9.77, 19.5, 39.1, 78.1, 156, 313, 625 and 1250 µg/plate (TA98: Main test)
0, 19.5, 39.1, 78.1, 156, 313, 625, 1250 and 2500 µg/plate (TA1537: Main test)
0, 1.22, 2.44, 4.88, 9.77, 19.5, 39.1 and 78.1 µg/plate (TA1535: Main test)
0, 39.1, 78.1, 156, 313, 625, 1250, 2500 and 5000 µg/plate (WP2uvrA/pKM101: Main test 2)
With S9 mix
0, 2.29, 6.86, 20.6, 61.7, 185, 556, 1667 and 5000 µg/plate (all strains: Dose determination test)
0, 39.1, 78.1, 156, 313, 625, 1250 and 2500 µg/plate (TA100, TA1535: Main test)
0, 78.1, 156, 313, 625, 1250, 2500 and 5000 µg/plate (WPurvA/pKM101: Main test)
0, 9.77, 19.5, 39.1, 78.1, 156, 313 and 625 µg/plate (TA98: Main test)
0, 39.1, 78.1, 156, 313, 625,1250 and 2500 µg/plate (TA1537: Main test) - Vehicle / solvent:
- Solvent used: DMSO
- Justification for choice of solvent/vehicle:
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- no
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- no
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- no
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not examined
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not examined
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
Any other information on results incl. tables
Growth inhibition of the bacterial lawn with the chemical (toxicity) was observed in S. typhimurium TA100, TA1535, TA98 and TA1537, with or without metabolic activation.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive Genotoxic
positive Cytotoxic
Based on these results, diallyl isophthalate was assessed to be mutagenic (positive) in the reverse mutation test in bacteria under the test conditions.
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