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EC number: 228-565-0 | CAS number: 6295-57-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Remarks:
- in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1985-05-07 to 1985-05-21
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Only the translation of the study report is available. All scientific necessary information for evaluation is available. The study was performed according to OECD guideline 405.
Data source
Reference
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- (benzothiazol-2-ylthio)acetic acid
- EC Number:
- 228-565-0
- EC Name:
- (benzothiazol-2-ylthio)acetic acid
- Cas Number:
- 6295-57-4
- Molecular formula:
- C9H7NO2S2
- IUPAC Name:
- 2-(1,3-benzothiazol-2-ylsulfanyl)acetic acid
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): Praeparat EK, Acetic acid (2-benzothiazolylthione)
- Physical state: fine, white powder
- Purity: 100%
- Purity test date: n.a.
- Impurities (identity and concentrations): n.a.
- Lot/batch No.: n.a.
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- 0.1 g
- Duration of treatment / exposure:
- 1 hour
- Observation period (in vivo):
- 14 days
- Number of animals or in vitro replicates:
- 6 (3 males + 3 females)
- Details on study design:
- In order to assess the eye irrtating potential of the test substance, 0.1 g of the undiluted test item was applied into the conjunctival sac of the left eye of each rabbit. The untreated eye serves as control. The treated eye remains unwashed.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 1.78
- Max. score:
- 3
- Reversibility:
- not fully reversible within: 14 days
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0.44
- Max. score:
- 1
- Reversibility:
- fully reversible within: 96 hours
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- not fully reversible within: 14 days after treatment
- Remarks on result:
- other: In one animal a score of 2 was determined at day 14 after treatment. In one other animal the severity progessed up to score 4 at day 14 after treatment and it is not expected to be reversible within 21 days.
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0.88
- Max. score:
- 2
- Reversibility:
- not fully reversible within: 14 days
- Remarks on result:
- other: 14 days after treatment in one animal the score was still 1; in one other animal the iris could not be assessed due to the cornea opacity.
- Irritant / corrosive response data:
- In order to assess the eye irritating potential of the test substance, 0.1 g of the undiluted test item was applied into the conjunctival sac of the left eye of each rabbit. The untreated eye serves as control. The treated eye remains unwashed. The assessment of possible effects to the eyes was performed with the assistance of a lamp 60 minutes, 24, 48, 72 and 96 hours after application of test item and additionally after 7 and 14 days due to some findings made. The eye reactions were assessed according to the numerical scoring system (acc. to Draize). The description of the degree and nature of irritation and the presence of serious damage were noted as follows:
No mortality or signs of systemic toxicity occurred.
Hyperemia of the conjunctiva was observed in all animals (score 1-2). In two of six animals this change was not reversible within the observation period of 14 days.
Chemosis of the conjunctiva (score1) was observed in all animals 24 hours after application. This effect was reversible within 72 hours.
Diffuse Corneal opacity areas (score 1, 2, 3) were observed in two of the six animals 48 hours after application and in all animals at 72 hours after application of the test substance. This observation was reversible in 4 animals by end of the 14-day observation period. The corneal opacity of the remaining two animals progressed up to complete opacity (score 4) in one case.
Hyperemia of the iris (score 1-2) was observed in 5 animals 48 hours after application and in all animals after 72 hours after application of the test substance. In one rabbit this observation was not reversible within the observation period and in a second rabbit the iris could not be evaluated due to the complete opacity of the cornea.
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (irreversible effects on the eye)
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Based on the results of this study the test substance has to be classified Category 1 "causes serious eye damage", as the damage was not reversible within the observation period of 14 days and is not expected to be reversible within 21 days.
- Executive summary:
In order to assess the eye irritating potential of the test substance, 0.1 g of the undiluted test item was applied into the conjunctival sac of the left eye of reach rabbit. The untreated eye serves as control. The treated eye remains unwashed. The assessment of possible effects to the eyes was performed with the assistance of a lamp 60 minutes, 24, 48, 72 and 96 hours after application of test item and additionally after 7 and 14 days due to some findings made. The eye reactions were assessed according to the numerical scoring system (acc. to Draize). The description of the degree and nature of irritation and the presence of serious damage were noted as follows:
- No mortality or signs of systemic toxicity occurred.
- Hyperemia of the conjunctiva was observed in all animals (score 1-2). In two of six animals this change was not reversible within the observation period of 14 days.
- Chemosis of the conjunctiva (score1) was observed in all animals 24 hours after application. This effect was reversible within 72 hours.
- Diffuse Corneal opacity areas (score 1, 2, 3) were observed in two of the six animals 48 hours after application and in all animals at 72 hours after application of the test substance. This observation was reversible in 4 animals by end of the 14-day observation period. The corneal opacity of the remaining two animals progressed up to complete opacity (score 4) in one case.
- Hyperemia of the iris (score 1-2) was observed in 5 animals 48 hours after application and in all animals after 72 hours after application of the test substance. In one rabbit this observation was not reversible within the observation period and in a second rabbit the iris could not be evaluated due to the complete opacity of the cornea.
Based on the results of this study the test substance has to be classified Category 1 "causes serious eye damage", as the damage was not reversible within the observation period of 14 days and is not expected to be reversible within 21 days.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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