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Diss Factsheets
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EC number: 200-589-6 | CAS number: 64-67-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1984
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Comparison ot ethanol toxicity to Daphnia magna and Ceriodaphnia dubia tested at two different temperatures: Static acute toxicity test results.
- Author:
- Takahashi, I.T.,Cowgill, U.M., Murphy, P.G.
- Year:
- 1 987
- Bibliographic source:
- Bull. Environ. Contam. Toxicol. 39:229-236.
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM E729-80
- Deviations:
- not specified
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Ethanol
- EC Number:
- 200-578-6
- EC Name:
- Ethanol
- Cas Number:
- 64-17-5
- Molecular formula:
- C2H6O
- IUPAC Name:
- ethanol
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- Analytical monitoring was not carried out as the authors estimated losses to be less than 10% over the course of the study.
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- TEST ORGANISM
Organisms were mass cultured an acclimated to temperature for at least 10 weeks and maintained in filtered, autoclaved Lake Huron water.
- Source: Source not specified
- Age at study initiation: Neonates.
- Common name: water flea
- Strain: Ceriodaphnia dubai
- Method of breeding: Neonates hatched by isolated gravid females gathered by sieving.
- Habitat change: alternate days
- Feeding during test
- Food type: bacteria free algae (A convolutus). Details on feeding reported in Cowgill (Env Toxicol Chem, 4, 415, 1985 and J Crust Biol 5, 420, 1985)
- Amount: 30000 (+/-2000) cells/ml
- feeding rate 3 mls algae/0.2litre vessel
- Frequency: alternate days
ACCLIMATION
- Acclimation period: 10 weeks
- Acclimation conditions (same as test or not): yes
OTHER
- dissolved oxygen: 8.3 +/- 0.2 mg/l
- pH: Initial: 8.2 (+/-0.1) Final 8.8 (+/-0.6)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 90.3 mg/l as CaCO3.(+/- 4.4)
- Test temperature:
- 24 to 24.9 degree C.
- pH:
- 8.2 (+/-0.1) to 8.4 (+/-0.1)
- Dissolved oxygen:
- 8.4 (+/-0.4) to 10.3 (+/-0.1)
- Salinity:
- not applicable
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 5 012 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: CI = 4233 - 5913
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- In a reliable 48 hour acute toxicity study carried out to an ASTM protocol, Ceriodaphnia dubai were exposed to ethanol at sufficient concentrations to enable an LC50 of 5012mg/l to be calculated based on an end point of mortality and as a geometric mean of 3 separate replicated assays. Based on the results of this study, ethanol would be not be classified toxic to the environment according to the classification system of the EU.
This toxicity study is classified as acceptable and satisfies the requirements as the key study for an invertebrate toxicity study.
The LC50 at 48 h was 5012mg/l
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