Ammonium undecafluorohexanoate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24 Apr - 26 Apr 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: control, 100 mg/L

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 100 mg of the test item was diluted in 0.5 L of dilution water (200 mg test item/L corresponding to 100 mg a.i./L). The solution was mixed in preparation containers and stirred to prepare the test solution.
- Eluate: no
- Differential loading: yes
- Controls: yes, test medium control

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain/clone: Clone 5
- Source: The University of Sheffield, UK (date of supply: 9 July 1990)
- Feeding during test: none
- Age: < 24 h

ACCLIMATION
- Age of parent animals: 20 d (survival rate: 100%)
- Acclimation conditions (same as test or not): same as test (dechlorinated tap water, water temperature (20 ± 1 °C) and photoperiod (16-hour light/8-hour dark))
- Type and amount of food: Chlorella vulgaris of 0.1 - 0.2 mg C (Organic carbon content)/day
- Feeding frequency: daily

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

40 mg/L (Ca, Mg)

Test temperature:

20.3 - 20.4 °C

pH:

7.7 - 7.8

Dissolved oxygen:

8.8 - 8.9 mg O2/L

Conductivity:

15 mS/m

Nominal and measured concentrations:

nominal: control, 100 mg a.i./L
measured: < LOD, 102 mg a.i./L (0 h), 101 mg a.i./L (48 h)

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beaker (volume: 100 mL)
- Type (delete if not applicable): covered with plastic lids
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water
- Total organic carbon: < 0.5 mg/L
- Particulate matter:
- Metals: < LOD
- Pesticides: < LOD
- Chlorine: < LOD
- Alkalinity: 39 mg/L
- Conductivity: 15 mS/m
- Culture medium different from test medium: no, same as test

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/8 h darkness
- Light intensity: room light

EFFECT PARAMETERS MEASURED
- Immobilisation was recorded every 24 h

RANGE-FINDING STUDY
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study: No immobilisation was recorded at a concentration of 100 mg/L. Thus, a limit test was performed.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: not observed
- Mortality of control: 0%
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: clear and colorless at test start and end

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Limit test: no
- Dose-response test: yes
- EC50 (48 h): 0.15 mg/L
- Other: EC50 value is within the rage of historical data of the laboratory.

The measured concentrations of the test item in the test solution were within the range of ±20% of the nominal concentration. Thus, the effect concentrations were based on the nominal values.

Validity criteria fulfilled:

yes

Description of key information

EC50 (48 h) > 100 mg a.i./L (nominal; OECD 202)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

One experimental study according to OECD 202 (GLP) is available investigating the short-term toxicity of ammonium undecafluorohexanoate (CAS 21615-47-4) to aquatic invertebrates. Based on the result from a preliminary range-finding test a limit test with a nominal concentration of 100 mg a.i./L was performed. The actual concentrations were analytically verified by HPLC/UV analysis and it was shown that the concentrations remained stable throughout the exposure (102 mg a.i./L at 0 h, 101 mg a.i./L at 48 h). No immobilization of Daphnia magna was recorded after 48 h. Thus, an EC50 (48 h) of > 100 mg a.i./L was derived.