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Diss Factsheets
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EC number: 480-880-4 | CAS number: 608-23-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Endpoint summary
Administrative data
Description of key information
The oral and dermal LD50 values in rats were > 2000 mg/kg bw in reliable studies according to OECD guidelines 425 and 402.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 3 Dec 2003 to 30 Dec 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 425 (Acute Oral Toxicity: Up-and-Down Procedure)
- Version / remarks:
- 2001
- GLP compliance:
- yes
- Test type:
- up-and-down procedure
- Limit test:
- yes
- Species:
- rat
- Strain:
- other: Outbred Albino
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Harlan, Indianapolis, IN
- Females (if applicable) nulliparous and non-pregnant: yes
- Age at study initiation: at least 49 days
- Weight at study initiation: 201.5-247.5 g
- Fasting period before study: yes
- Housing: individually
- Diet: TEK 7012 Rodent Diet, Harlan Teklad, Madison, WI, ad libitum
- Water: ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 +/- 2.8
- Humidity (%): 30-70
- Air changes (per hr): 10-15
- Photoperiod (hrs dark / hrs light): 12/12 - Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- MAXIMUM DOSE VOLUME APPLIED: 1 ml/100 g bw
- Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observations: twice daily on day of dosing, daily thereafter; weighing on day 0, 7, 14 and at death
- Necropsy of survivors performed: yes - Statistics:
- Statistical computer program (AOT425StatPgm) developed by the United States Environmental Protection Agency
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- Two out of five animals were found dead in the course of the study (Animal # 1 on Day 12 and Animal # 3 on Day 4). Three out of five animals survived the duration of the study
- Clinical signs:
- other: In four out of five animals, no clinical manifestations of toxicity were observed. In Animal # 3, piloerection was observed within 4 hours of test substance administration
- Gross pathology:
- No unusual findings were found during necropsy in all the dosed animals.
- Interpretation of results:
- Category 5 based on GHS criteria
- Remarks:
- toxicity presumably meets criteria for GHS category 5. A defnite conclusion is not possible based on the limit test. Cat 5 is not foreseen in EU CLP Regulation
- Conclusions:
- Under the conditions of this study, the oral LD50 in rats was > 2000 mg/kg bw
- Executive summary:
The test substance, 1-Chloro-2,3-dimethylbenzene, was evaluated for its acute toxcity following oral administration of 2000 mg/kg bw (limit dose) to five female rats. Two deaths were observed in this study. One animal showed clinical signs of toxicity (piloerection). Using OECD guideline 425 as limit test, the test substance was defined to have an estimated LD50 of greater than 2000 mg/kg body weight.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- discriminating dose
- Value:
- 2 000 mg/kg bw
- Quality of whole database:
- Key study is reliable without restrictions
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- other:
Reference
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 17 Dec 2003 to 31 Dec 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Millbrook Breeding Labs, Amherst, MA, USA
- Females (if applicable) nulliparous and non-pregnant: yes
- Age at study initiation: 12-15 weeks
- Weight at study initiation: 2.00-2.36 kg
- Fasting period before study: no
- Housing: individually
- Diet: TEK 8630 Rabbit Diet, Harlan Teklad, Madison, WI, USA, ad libitum
- Water: ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 +/- 3
- Humidity (%): 30-70
- Air changes (per hr): 10-15
- Photoperiod (hrs dark / hrs light): 12/12 - Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure: approx. 10 % of body surface
REMOVAL OF TEST SUBSTANCE
- Washing (if done): with USP water for injection
- Time after start of exposure: 24 h
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2000 mg/kg bw - Duration of exposure:
- 24 h
- Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- not required
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observations: frequently during the first day, careful clinical observations at least once daily; weighing: days 0, 7 and 14
- Necropsy of survivors performed: Yes - Statistics:
- not performed
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No effects
- Clinical signs:
- other: No effects
- Gross pathology:
- No effects
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Under the conditions of this study, the dermal LD50 in rabbits was > 2000 mg/kg bw.
- Executive summary:
The test substance, 1-chloro-2,3-dimethylbenzene, was evaluated for its potential to produce systemic toxicity or death after a single topical 24-hour application to the skin of albino rabbits at a dose of 2000 mg/kg bw. No animal died during the course of the study, therefore the test substance is considered non-toxic according to the criteria of the study protocol.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- discriminating dose
- Value:
- 2 000 mg/kg bw
- Quality of whole database:
- Key study is reliable without restrictions
Additional information
The test substance, 1-chloro-2,3-dimethylbenzene, was evaluated for its acute toxicity following oral administration of 2000 mg/kg bw to five female rats. Two deaths were observed in this study. One animal showed clinical signs of toxicity (piloerection). Using the OECD guideline 425 as limit test, the test substance was defined to have an estimated LD50 of greater than 2000 mg/kg bw. This result is supported by a less reliable study.
The test substance, 1-chloro-2,3-dimethylbenzene, was evaluated for its acute toxicity after a single topical 24-hour application to the skin of albino rabbits at a dose of 2000 mg/kg bw. No animal died during the course of the study, therefore the test substance is considered non-toxic according to the criteria of the study protocol. This result is supported by a less reliable study.
Justification for classification or non-classification
Due to LD50 values of > 2000 mg/kg bw for oral and dermal exposure, no classification for acute toxicity is required according to the criteria of Regulation (EC) No 1272/2008.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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