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EC number: 224-581-7 | CAS number: 4418-61-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08.01. – 26.01.2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- Daphnia sp. Acute Immobilisation Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- The determination of the test item concentrations was carried out by HPLC method.
Preliminary test: The analytical determination of the test item concentrations were performed at the beginning of the test and at the end of the test. The samples for analysis were taken from the highest (100 mg·L-1) and the lowest test concentration (1 mg·L-1). The samples for analysis (0 hours) were prepared at the beginning of the test and immediately delivered in transport box to analytical laboratory. The samples were analysed on the day of delivery. The samples for analysis at the end of the test (48 hours) were delivered to analytical laboratory immediately after the end of testing. The samples were analysed on the day of delivery. All samples were stored at laboratory temperature.
The analytical results showed, that the test item was stable in dilution water at the conditions of the test.
Definitive test: The analytical determination of the test item concentrations were performed at the beginning of the test and at the end of the test. The samples for analysis were taken from the highest (100 mg·L-1) and the lowest (3 mg·L-1) test concentrations. The samples for analysis (0 hours) were prepared at the beginning of the test and immediately delivered in transport box to analytical laboratory. The samples were analysed on the day
of delivery. The samples for analysis at the end of the test (48 hours) were delivered to analytical laboratory immediately after the end of testing. The samples were analysed on the day of delivery. All samples were stored at laboratory temperature. - Details on test solutions:
- The stock solutions of the test item were prepared in the dilution water. 50 mg of the test item was weighed into 500 mL of the dilution water for the preliminary test and 100 mg of the test item was weighed into 1000 mL of the dilution water for the definitive test.
The concentrations of solutions used in the preliminary and definitive test were obtained by dilution of the stock solution with dilution water. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna Straus were obtained by aperiodic parthenogenesis from own laboratory breeding. Daphnia were fed by a mixture of algae. The sensitivity of the test species is periodically monitored by testing with the reference substance, potassium dichromate.The grow-up gravid females are separated into dilution water. Young daphnia born in 24 hours are segregated to the handling container from which they entered the experiment. At handling a care should be taken of not to penetrate the air under animal’s shell.
TEST ORGANISM
- Common name: Daphnia Magna
- Strain: Straus
- Source: own laboratory breeding
- Age at study initiation: young daphnia born in 24 hours
- Method of breeding: aperiodic parthenogenesis
- Feeding during test: no
ACCLIMATION
The grow-up gravid females are separated into dilution water. Young daphnia born in 24 hours are segregated to the handling container from which they entered the experiment.
- Type and amount of food: a mixture of algae
- Health during acclimation: no mortality observed - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 2.53 mmol/L
The content of sum Ca + Mg ions should have the value of 2.50 +/- 0.10 mmol/L. - Test temperature:
- 20.0 ± 2.0 °C; with a variation of ± 1°C at each individual test
- pH:
- 7.8
- Dissolved oxygen:
- 8.9 mg·L-1
- Conductivity:
- 1.13 µS·cm-1
- Nominal and measured concentrations:
- 3, 5, 9, 17,31, 56, 100 mg/L + C(control)
Determined concentration of test substance
0 hours: 3.01 ; 100.9 mg·L-1
48 hours: 3.10 ; 105.0 mg·L-1 - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers 50ml
- Type (delete if not applicable): open
- Aeration: no
- Lighting: daylight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: at least 2 ml per individual
For holding and culturing of the daphnia the M4 medium was used (according to Annex 2 of the EU method C.2 and according to Annex 3 of the OECD TG202).
For test was used dilution water (according to Annex 2 of the EU method C.2 and according to Annex 3 of the OECD TG 202).
Dilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D into 1000 mL of deionized water.
Because culturing and test medium was different we included pre-test acclimation period: brood daphnids were maintained in dilution water for 48 hours prior to the start of the test.
A. 117.6 g CaCl2.2H20 in 1 L of deionized water
B. 49.3 g MgSO4.7H2O in 1 L of deionized water
C. 25.9 g NaHCO3 in 1 L of deionized water
D. 2.3 g KCl in 1 L of deionized water - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 50.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 20.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- Reference test
The sensitivity of the test species and correctness of test performance is periodically verified in six-month period by testing with the reference item, potassium dichromate. The results of the verification test with K2Cr2O7, carried out in period from 06.12. to 08.12.2017 are the following:
24 hour – EC50 = 1.10 mg·L-1 (95% confidence limit: 0.96 – 1.28 mg·L-1)
48 hour – EC50 = 0.77 mg·L-1 (95% confidence limit: 0.69 – 0.86 mg·L-1) - Validity criteria fulfilled:
- yes
- Conclusions:
- There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test item has been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, the results can be based on nominal or measured initial values.
The nominal concentrations were used for all evaluation and results.
Test results:
24 hour – EC50 = 50.7 mg·L-1 (nominal concentration) (95% confidence limit: 34.2 – 92.3 mg·L-1)
48 hour – EC50 = 20.4 mg·L-1 (nominal concentration) (95% confidence limit: 14.3 – 29.9 mg·L-1) - Executive summary:
The test item, 5-aminotetrazole, was tested in acute immobilisation test on Daphnia magna.
The test was performed according to method C.2 – Daphnia sp. Acute Immobilisation Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
The preliminary test was performed in a range of the test item nominal concentrations 1 – 100 mg·L-1. The test was performed as static.
Based on toxicity of the test item found in the preliminary test, the definitive test was performed in appropriate concentration range.
The analytical results showed, that the test item 5-aminotetrazole was sufficiently stable in dilution water in the conditions of the test, therefore the definitive test was performed as static one.
The definitive test was performed in a range of the test item nominal concentrations 3 – 100 mg·L-1. Samples for analytical determination were taken at the beginning and at the end of the test.
There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test item has been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, the results can be based on nominal or measured initial values.
The nominal concentrations were used for all evaluation and results.
Test results:
24 hour – EC50 = 50.7 mg·L-1 (nominal concentration)
(95% confidence limit: 34.2 – 92.3 mg·L-1)
48 hour – EC50 = 20.4 mg·L-1 (nominal concentration)
(95% confidence limit: 14.3 – 29.9 mg·L-1)
24 hour – EC0 = 3 mg·L-1 (nominal concentration)
48 hour – EC0 = 3 mg·L-1 (nominal concentration)
24 hour – EC100 > 100 mg·L-1 (nominal concentration)
48 hour – EC100 > 100 mg·L-1 (nominal concentration)
Reference
Description of key information
The test substance was tested in acute immobilisation test on Daphnia magna according to method C.2 – Daphnia sp. Acute Immobilisation Test (GLP study). The test was performed as static.
24 hour–EC50 = 50.7 mg·L-1 (nominal concentration)
48 hour–EC50 = 20.4 mg·L-1 (nominal concentration)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 50.7 mg/L
Additional information
Because concentrations of the test substance have been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, nominal concentrations were used.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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