Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 814-059-2 | CAS number: 68680-98-8
Ecotoxicological information
Toxicity to aquatic algae and cyanobacteria
Currently viewing:
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was performed according to German national standard guidelines (no GLP, no analytics).
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Reference
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was performed according to German national standard guidelines (no GLP, no analytics).
- Justification for type of information:
- See read-across justification attached in section 13.2
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38 412, L9
- GLP compliance:
- no
- Analytical monitoring:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 1000 mg/l was prepared. The stock solution was prepared by dissolving 1000 mg test substance and 100 mg of the solubilizer Cremophor in dilution water. The test concentrations were then prepared by dilution with test water.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): 25 mg Cremophor/l
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 0.5, 1.0, 2.5, 5.0, 10.0, 25.0 mg/l - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: SAG 86.81
- Source (laboratory, culture collection): Algae collection Goettingen - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 21.3 °C
- pH:
- 7.75 - 7.99
- Nominal and measured concentrations:
- Nominal test concentration (mg/l):
0 (control), 50, 10, 25, 50, 100, and 250 - Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: 4
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to DIN 38412 L9
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: At 0, 24, 48, 72, and 96 hours after test start, the algae cell density was determined by spectro-fluorometrical measurement using an impulse fluorometer. The parameters pH, and temperature were determined at the end of the test. Also, at the end of the incubation period the algae cells were analyzed for signs of photosynthesis blockage by means of a spectrophotometrical scan at 300 - 780 nm. - Reference substance (positive control):
- yes
- Remarks:
- Potassium chromate p.a.
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 21.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 9.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Reported statistics and error estimates:
- The EC50 values were calculated according to the procedure of Tallaradia & Jacob (1979): The dose-response relation in pharmacology. Springer Verlag, 98-103.
Nominal test fluorometer values after x hours
concentration
(mg/l) 0 / 24 / 48 / 72 / 96 /
-----------------------------------------------
S.Control / 12 / 43 / 117 / 370 / 799 /
Control / 12 / 45 / 122 / 357 / 762 /
5.0 / 13 / 44 / 104 / 273 / 649 /
10.0 / 13 / 43 / 101 / 255 / 639 /
25.0 / 13 / 41 / 91 / 216 / 530 /
50.0 / 13 / 32 / 38 / 40 / 57 /
100.0 / 12 / 18 / 15 / 13 / 11 /
250.0 / 11 / 6 / 4 / 4 / 4 /
S.Control = solvent control
*10000 cells/ml were introduced at the test start.
ECx values (in mg/l) after x hours of exposure
/ 72 h / 96 h /
EBC10 / 7.462 / 6.340 /
EBC50 / 18.581 / 15.676 /
ErC10 / 9.457 / 16.021 /
ErC50 / 21.624 / 25.887 /
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38 412, L9
- GLP compliance:
- no
Test material
- Reference substance name:
- 3,7-dimethyloctan-3-ol
- EC Number:
- 201-133-9
- EC Name:
- 3,7-dimethyloctan-3-ol
- Cas Number:
- 78-69-3
- Molecular formula:
- C10H22O
- IUPAC Name:
- 3,7-dimethyloctan-3-ol
- Details on test material:
- - Name of test material (as cited in study report): Tetrahydrolinalool
- Lot/batch No.: 36-0589
Purity: no data
Water solubility: 320 mg/l at 25 °C
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 1000 mg/l was prepared. The stock solution was prepared by dissolving 1000 mg test substance and 100 mg of the solubilizer Cremophor in dilution water. The test concentrations were then prepared by dilution with test water.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): 25 mg Cremophor/l
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 0.5, 1.0, 2.5, 5.0, 10.0, 25.0 mg/l
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: SAG 86.81
- Source (laboratory, culture collection): Algae collection Goettingen
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21.3 °C
- pH:
- 7.75 - 7.99
- Nominal and measured concentrations:
- Nominal test concentration (mg/l):
0 (control), 50, 10, 25, 50, 100, and 250 - Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: 4
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to DIN 38412 L9
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: At 0, 24, 48, 72, and 96 hours after test start, the algae cell density was determined by spectro-fluorometrical measurement using an impulse fluorometer. The parameters pH, and temperature were determined at the end of the test. Also, at the end of the incubation period the algae cells were analyzed for signs of photosynthesis blockage by means of a spectrophotometrical scan at 300 - 780 nm. - Reference substance (positive control):
- yes
- Remarks:
- Potassium chromate p.a.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 21.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 9.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Reported statistics and error estimates:
- The EC50 values were calculated according to the procedure of Tallaradia & Jacob (1979): The dose-response relation in pharmacology. Springer Verlag, 98-103.
Any other information on results incl. tables
Nominal test fluorometer values after x hours
concentration
(mg/l) 0 / 24 / 48 / 72 / 96 /
-----------------------------------------------
S.Control / 12 / 43 / 117 / 370 / 799 /
Control / 12 / 45 / 122 / 357 / 762 /
5.0 / 13 / 44 / 104 / 273 / 649 /
10.0 / 13 / 43 / 101 / 255 / 639 /
25.0 / 13 / 41 / 91 / 216 / 530 /
50.0 / 13 / 32 / 38 / 40 / 57 /
100.0 / 12 / 18 / 15 / 13 / 11 /
250.0 / 11 / 6 / 4 / 4 / 4 /
S.Control = solvent control
*10000 cells/ml were introduced at the test start.
ECx values (in mg/l) after x hours of exposure
/ 72 h / 96 h /
EBC10 / 7.462 / 6.340 /
EBC50 / 18.581 / 15.676 /
ErC10 / 9.457 / 16.021 /
ErC50 / 21.624 / 25.887 /
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
