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EC number: 433-470-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 8 January 2004
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The study includes data generated according to generally valid and internationally accepted testing guidelines and performed according to GLP. The study was based on: -OECD Guidelines for Testing of Chemicals, Section 2: Effects on biotic systems, Guideline no. 209, "Activated Sludge, Respiration Inhibition Test", adopted April 4, 1984. - EEC Directive 67/548 amended November 18, 1987 (87/302), Part C: Methods for the determination of ecotoxicity, Publication No. L 133, "Biodegradation: Activated sludge respiration inhibition test", adopted May 30, 1988.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- GLP Statement date: 27/01/2004
- Analytical monitoring:
- not required
- Vehicle:
- yes
- Details on test solutions:
- TEST SUBSTANCE - PREPARATION AND TEST CONDITIONS
Since Setafix X 11342 was poorly soluble in water, the test substance was quantitatively added to the test vessels. A nominal concentration of 100 mg/I was tested in duplicate. The concentration was approved by the study director in the study files. Due to the test set-up (continuous aeration and stirriing) optimal contact between the test substance and test medium was ensured. - Test organisms (species):
- other: Micro-organisms in activated sludge
- Details on inoculum:
- -Source:
Municipal sewage treatment plant: 'Waterschap de Maaskant', 's-Hertogenbosch, the Netherlands, receiving predominantly domestic sewage.
- Number of micro-organisms:
Number of micro-organisms was determined as the amount of Mixed Liquor Suspended Solids (MLSS) per litre test medium.
- Preparation of the sludge:
The sludge was coarsely sieved and washed with tapwater. A small amount of the sludge was weighed and dried at ca. 105°C to determine the amount of suspended solids (3.6 g/I of sludge, as used for the test). The pH was 7.5. The batch of sludge was used on the subsequent day, therefore 50 ml of synthetic sewage feed was added to each litre of activated sludge at the end of the day. The sludge was kept aerated at test temperature until use.
- Rationale:
Recognized by international guidelines as the recommended test system. - Test type:
- other: Continuous aeration and stirring
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
- Remarks on exposure duration:
- During which aeration and stirring took place.
- Post exposure observation period:
- 30 minutes
- Hardness:
- Not specified
- Test temperature:
- 19.4 - 19.6 °C
- pH:
- 7.3
- Dissolved oxygen:
- 7.7 - 8.0
- Salinity:
- Not specified
- Nominal and measured concentrations:
- Nominal Concentration: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: All glass, 300 ml oxygen bottles and 1 L test bottles.
- Aeration: Clean, oil-free air
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Milli-RO I Milli-Q water: Tap-water purified by reverse osmosis {Milli-RO) and subsequently passed over activated carbon and ionexchange cartridges {Milli-Q) {Millipore Corp., · Bedford, Mass., USA).
- Synthetic sewage feed:
16 g peptone, 11 g meat extract, 3 g urea, 0.7g NaCl, 0.4 g CaCl2.2H20, 0.2 g MgS04.?H20, 2.8 g K2HP04, Dissolved in Milli-Q water, made up to 1 I and filtered, The pH was 7.0.
- Oxygen meter
WTW inolab Oxi Level 2 supplied with a WTW CellOx 325 oxygen electrode, electrolyte type ELY/G.
PERFORMANCE OF THE TEST
The synthetic sewage feed (16 ml) and an adequate amount of the test substance were mixed and made up to 300 ml with Milli-RO water. Activated sludge (200 ml) was added to provide a final volume of 500 ml. The mixture was then aerated in a 1 L bottle during the contact time, using a pipette as an aeration device.
Then a well mixed sample of the contents was poured into a 300 ml oxygen bottle, and the flask was sealed with an oxygen electrode connected to a recorder, forcing the air out of the vessel. Oxygen consumption was measured and recorded for approximately 10 minutes. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer.
The pH was determined in the remaining part of the reaction mixture. Temperature was determined in the remaining part of the reaction mixture of the first control and first test substance vessel.
This procedure was repeated for the duplicate concentration. In each test series two controls without test substance were tested, one at the start and one at the end. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 30 min
- Dose descriptor:
- other: No inhibition of respiration rate of the sludge was recorded at 100 mg Setafix X 11342 per litre
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- No inhibition of respiration rato of the sludge was recorded at 100 mg Setafix X 11342 per litre. The duplicate measurement confirmed the result of the first measurement. Therefore no further testing was needed.
- Results with reference substance (positive control):
- The EC50 of 3,5-dichlorophenol was in the accepted range of 5-30 mg/I (10 mg/I, regression line: Y = 70.95 X-21.53, Y = % inhibition and X =log concentration (mg/I).
- Validity criteria fulfilled:
- yes
- Remarks:
- All criteria for acceptability of the test were met
- Conclusions:
- In conclusion, under the conditions of this present test, Setafix X 11342 was not toxic to waste water (activated sludge) bacteria at a concentration of 100 mg/L.
- Executive summary:
The principal objective of this study was the assessment of the effect of a test substance on micro-organisms by measuring the respiration rate under defined conditions in the presence of different concentrations of test substance Setafix X 11342. The test method is a rapid scrnening method whereby test substances which may adversely affect aerobic microbial treatment plants can be identified. The study procedure was based on OECD Guideline No. 209, adopted April 4, 1984 and EEC Directive 67/548 amended November 18, 1987 (87/302), Part C, Publication No. L 133, adopted May 30, 1988.
The influence of Setafix X 11342 on the respiration rate of activated sludge was investigated after a contact time of 30 minutes. Setafix X 11342 was a light yeillowish solid. Since Setafix X 11342 was poorly soluble in water, the test substance was quantitatively added to the test vessels. A nominal concentration of 100 mg/L was tested in duplicate. Due to the test set-up (continuous aeration and stirring) optimal contact between the test substance and test medium was ensured.
No inhibition of respiration rate of the sludge was recorded at 100 mg Setafix X 11342 per litre. The duplicate measurement confirmed the result of the first measurement. Therefore no further testing was needed.
Since all criteria for acceptability of the test were met, this study was considered to be valid.
In conclusion, under the conditions of this present test, Setafix X 11342 was not toxic to waste water (activated sludge) bacteria at a concentration of 100 mg/L.
Reference
Description of key information
No inhibition of respiration rate of the sludge was recorded at 100 mg
Setafix X 11342 per litre. The duplicate measurement confirmed the
result of the first measurement. Therefore no further testing was needed.
In conclusion, under the conditions of this present test, Setafix X
11342 was not toxic to waste water (activated sludge) bacteria at a
concentration of 100 mg/L.
Key value for chemical safety assessment
Additional information
The principal objective of this study was the assessment of the effect of a test substance on micro-organisms by measuring the respiration rate under defined conditions in the presence of different concentrations of test substance Setafix X 11342. The test method is a rapid screening method whereby test substances which may adversely affect aerobic microbial treatment plants can be identified. The study procedure was based on OECD Guideline No. 209, adopted April 4, 1984 and EEC Directive 67/548 amended November 18, 1987 (87/302), Part C, Publication No. L 133, adopted May 30, 1988.
The influence of Setafix X 11342 on the respiration rate of activated sludge was investigated after a contact time of 30 minutes. Setafix X 11342 was a light yellowish solid. Since Setafix X 11342 was poorly soluble in water, the test substance was quantitatively added to the test vessels. A nominal concentration of 100 mg/L was tested in duplicate. Due to the test set-up (continuous aeration and stirring) optimal contact between the test substance and test medium was ensured.
No inhibition of respiration rate of the sludge was recorded at 100 mg Setafix X 11342 per litre. The duplicate measurement confirmed the result of the first measurement. Therefore no further testing was needed.
Since all criteria for acceptability of the test were met, this study was considered to be valid.
In conclusion, under the conditions of this present test, Setafix X 11342 was not toxic to waste water (activated sludge) bacteria at a concentration of 100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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