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EC number: 812-958-4 | CAS number: 1078712-76-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 27, 2016 to September 16, 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- GLP compliance:
- yes
Test material
- Reference substance name:
- Reference substance 001
- Cas Number:
- 1078712-76-1
- Molecular formula:
- C23-27H49-59N (Substance is a UVCB)
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge collected from the Easton Wastewater Treatment Facility, Easton, Maryland on
July 27, 2016 was utilized as the inoculum for the test. The Easton facility receives wastes from
predominately domestic sources. The sludge was sieved using a 2 mm screen and allowed to settle for
30 minutes. The supernatant above the settled solids was removed and the total suspended solids (TSS)
concentration of the settled sludge was determined. Total suspended solids in the settled sludge were
adjusted to a nominal concentration of approximately 3000 mg/L by dilution with municipal water. Fifty
mL of synthetic sewage (Appendix I of Protocol) was added to each liter of adjusted sludge. The sludge
was maintained at a temperature of 20 2C and continuously aerated overnight. The pH and total
suspended solids concentration of the activated sludge were determined on the day of use in the study.
Study design
- Test type:
- static
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Details on test conditions:
- Test mixtures were prepared at 15 minute intervals starting with the first control. The control
contained 16.0 mL of synthetic sewage, 234 mL of municipal water, and 250 mL of inoculum for a total
volume of 500 mL. The mixture was promptly aerated at a rate sufficient to keep the solids in suspension.
Subsequent reference and test substance dosed biotic mixtures were prepared by combining 16.0 mL of
synthetic sewage, the appropriate volume of reference substance stock solution or appropriate amount of
test substance, and enough municipal water needed to achieve a volume of 250 mL was added. After the
municipal water was added, 250 mL of inoculum was added to bring the total volume up to 500 mL.
The abiotic mixture contained 16.0 mL of synthetic sewage, 0.5000 g of test substance, and 484 mL
of municipal water for a final volume of 500 mL. Finally, a second control was prepared in the same
manner as the first control. All mixtures were aerated for three hours.
Results and discussion
Effect concentrations
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- EC50 of 22.6 mg/L with 3,5-dichlorophenol
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
The potential effect of Amines, (2-ethylhexyl)(hydrogenated tallow alkyl)methyl (from this point
on referred to Amines MHTEH) on activated sludge microorganisms maintained in an aerobic
environment was assessed by the Activated Sludge Respiration Inhibition Test Method (OECD Guideline
209). The test contained control, reference and treatment groups. The control replicates were used to
determine the background respiration rate of the sludge and were not dosed with the test or reference
substance. The reference group was dosed with 3,5-dichlorophenol, a known inhibitor of respiration, at
concentrations of 3, 15 and 50 mg/L. The treatment group was dosed with Amines MHTEH at
concentrations of 10, 100, and 1000 mg/L. The 1000 mg/L treatment was tested in triplicate. An abiotic
control was dosed with the test substance at a concentration of 1000 mg/L to discriminate between abiotic
uptake by the test substance and microbial respiration. After an exposure period of three hours, the
respiration rates of the test solutions were measured using an YSI Model 5000 Dissolved Oxygen Meter.
The respiration rates in the two controls were 29.6 and 27.2 mg O2/L/hr. Both controls had
respiration rates above 26.87 mg O2/L/hr, which is the low limit based on the validity criteria for mixtures
with 1.34 g dry weight of suspended solids per liter. The coefficient of variation of the two control
respiration rates was 5.9%, and was within the 30% limit established for the test. The validity of the test
was further supported by the results from the 3,5-dichlorophenol reference group, which resulted in an
EC50 value of 22.6 mg/L, with 95 percent confidence limits of 15 mg/L and 50 mg/L. The EC50 for the
reference substance was within the 2 to 25 mg/L range considered acceptable for the test.
An inhibitory dose response effect was not observed for the 10 mg/L, 100 mg/L, or 1000 mg/L
treatment groups. The EC50 value for Amines MHTEH is greater than 1000 mg/L, the highest
concentration tested. The abiotic treatment mixture dosed with 1000 mg/L of Amines MHTEH had a
respiration rate of 0.0 mg O2/L/hr showing there was no significant uptake or release of oxygen resulting
from abiotic reactions of the test substance.
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