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EC number: - | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- (Q)SAR
- Adequacy of study:
- weight of evidence
- Study period:
- 2017
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification
- Justification for type of information:
- 1. SOFTWARE
EPIsuite
2. MODEL (incl. version number)
BIOWIN (v4.10)
3. SMILES OR OTHER IDENTIFIERS USED AS INPUT FOR THE MODEL
O=P(CC)(CC)OC(C)CO - Principles of method if other than guideline:
- - Software tool(s) used including version:
EPIsuite V.4.0
- Model(s) used:
BIOWIN (v4.10)
- Model description: Biowin3 (Survey Model - Ultimate Biodeg) and Biowin5 (MITI Linear Biodeg Probability) - Key result
- Parameter:
- probability of ready biodegradability (QSAR/QSPR)
- Remarks:
- Ready Biodegradability Prediction: NO
- Remarks on result:
- not measured/tested
- Conclusions:
- Ready Biodegradability Prediction: NO
- Executive summary:
Ready Biodegradability Prediction: NO
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- September- December 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- All three deviation are considered as "uncritical concerning the outcome of the study."
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Activated sludge from a biologic sewage treatment plant was used as inoculum. The chosen plant treats mostly domestic sewage.
The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, 67435 NW-Lachen-Speyerdorf.
Date of collection: 31. Oct. 2019, batch no: E20191031.
The sludge was filtrated, washed with test medium (2x) and re-suspended in test medium. It was then aerated until use. The dry matter was determined to contain 3.10 g of suspended solids/L. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- Flask volume 1500 mL
Apparatus blanks 2, containing mineral medium only
Blank Controls 2, containing mineral medium and inoculum
Positive control flasks 2, containing positive control, mineral medium and inoculum
Test flasks 2, containing test item, mineral medium and inoculum
Abiotic control 1, containing test item, mineral medium and HgCl2
Toxicity control 1, containing test item, positive control, mineral medium and in-oculum
Inoculum concentration: 25.0 mg/L
Temperature 19.2 – 21.1 °C without direct lighting
Duration 28 days
The test was performed with a nominal start concentration of 20 mg organic carbon/L of the test item. - Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 4
- Sampling time:
- 28 d
- Details on results:
- 10-day-window: not detected
degradation at the end of 10-day-window none
degradation at the end of the test 4 %
pass level following guideline: 60 % at the end of 10-day-window for pure substances
respective 60 % at the end of the test for mixtures - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Applying the 10-day-window, the test item E17-194T is not readily biode-gradable following OECD 301B and EU C.4-C respectively.
- Executive summary:
Applying the 10-day-window, the test item E17-194T is not readily biode-gradable following OECD 301B and EU C.4-C respectively.
Referenceopen allclose all
Description of key information
QSAR assessment:
Ready Biodegradability Prediction: NOExperimental results:
Applying the 10-day-window, the test item E17-194T is not readily biode-gradable following OECD 301B and EU C.4-C respectively.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
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