Registration Dossier
Registration Dossier
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EC number: 701-365-5 | CAS number: -
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The test item is found not harmful to activated sludge. The EC50 lies above 10000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 000 mg/L
Additional information
In GLP compliant tests the test substance was assessed by following OECD Guideline 209. On exposing the activated sludge to test substance for 3 h at several concentrations respiratory inhibition was measured. The results from the two studies indicate the 3 h EC50 value to be greater than 5000 or 2000 mg/l respectively.
The test was conducted with Reactive Black 5 which easily degrades to Reactive Black 5 bis-vinyl in the presence of water. The inoculum was 1.6 g/L; the graph of the dose-effect curve shows that there is no dose-dependency at concentrations of 2500, 5000 and 10000 mg/L.
Due to the nearly parallel curve progression to the "concentration axis" it is assumed that there is definitely no inhibition up to a concentration of 5000 mg/L and most likely no inhibition at a concentration of 10000 mg/L.
Hence, Reactive Black 5 is not harmful to activated sludge. The EC50 lies above 10000 mg/LInformation on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
