N,N-bis(1-methylethyl)formamide

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From January 25, 2021 to February 23, 2021

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

17th July 1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Batch number: M-O303190723301F
Purity: 99.68%

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): from the aeration tank of Shenyang North Sewage Treatment Plant 1 day before the test exposure was started.
- Pretreatment: coarse particles were removed by filtration through a fine sieve and the sample was settled. After the supernatant was discarded, the sludge was washed twice with mineral medium and the concentrated sludge was suspended in mineral medium.
- Concentration of sludge: The measured concentration of suspended sludge was 5.515 g/L as suspended solids (SS).
- Initial cell/biomass concentration: 30 mg/L (as suspended solids (SS))

Duration of test (contact time):

28 d

Initial conc.:

37.48 mg/L

Based on:

test mat.

Details on study design:

TEST SYSTEM
- Culturing apparatus: 250 mL flask
- Number of culture flasks/concentration: 2 replicates
- Method used to create aerobic conditions:
- Measuring equipment: Oxygen uptake was measured by BOD analyzer.

CONTROL AND BLANK SYSTEM
- Inoculum blank: Test vessels containing only 250 mL inoculated mineral medium were prepared in duplicate and served as inoculum controls.
- Abiotic sterile control:
- Toxicity control: Toxicity control test vessel contained both test item and reference item.
- Other:

Reference substance:

benzoic acid, sodium salt

Key result

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

Details on results:

Based on the percentage biodegradation expressed as average, the percentage biodegradation of test item after 28 days averaged 0.0% (2 replicates). The percentage degradation of the test item did not reach the pass level of 60% after 28 days of the test.

Results with reference substance:

Biodegradation of the reference substance (Sodium benzoate) reached 61.4% after 3 day and 17 hours, and was 78.1% after day 14, and 84.0% after day 28.
In the Toxicity Control (TC) test mixture, 38.3% degradation occurred within 14 days and the value exceeded 25% based on total ThOD, which indicated that the test item can be assumed to be not inhibitory under the conditions of this test.

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test item is not readily biodegradable under the described test conditions.

Executive summary:

The ready biodegradability test of the test item was conducted with unacclimatised sewage micro-organism by measuring oxygen consumption over a 28-day test period, according to OECD Guideline 301F under GLP.

Based on the percentage biodegradation expressed as average, the percentage biodegradation of test item after 28 days averaged 0.0% (2 replicates). The percentage degradation of the test item did not reach the pass level of 60% after 28 days of the test. The test item is not readily biodegradable under the described test conditions.

Description of key information

The ready biodegradability test of the test item was conducted with unacclimatised sewage micro-organism by measuring oxygen consumption over a 28-day test period, according to OECD Guideline 301F under GLP.

Based on the percentage biodegradation expressed as average, the percentage biodegradation of test item after 28 days averaged 0.0% (2 replicates). The percentage degradation of the test item did not reach the pass level of 60% after 28 days of the test. The test item is not readily biodegradable under the described test conditions.

Key value for chemical safety assessment

Biodegradation in water:

not biodegradable

Type of water:

freshwater