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Diss Factsheets
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EC number: 446-560-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 May to 20 May 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: Commission Directive 88/302/EEC; Official Journal of the EG L133 Part C (1988)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct weighing into test flasks
- Controls: Yes, blank control
- Test concentration separation factor: 1.8 - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: mixed population of aquatic microorganisms(activated sludge)
- Name and location of sewage treatment plant where inoculum was collected: aeration tank of a domestic sewage treatment plant(Wupper area water authority)
- Method of cultivation: Stirring period of test item before start of incubation time: 16 h.
- Initial biomass concentration: 480 mg/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 +/- 2 °C
- Nominal and measured concentrations:
- 10, 18, 32, 56, 100 mg/L as nominal concentrations of the test item.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Flask
- Aeration: Permanent aeration
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2 controls
- Sludge concentration (weight of dry solids per volume): 480 mg/L suspended solids
- Nutrients provided for bacteria: Daily feed with synthetic medium
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : respiration inhibition at 3 h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 44 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium: The solubility was not exceeded.
- Blank controls oxygen uptake rate: 34 mg/L - Results with reference substance (positive control):
- - Results with reference substance valid? Yes, all validity criteria are fulfilled.
- Relevant effect levels: 0 % inhibition at 2.5 mg/L, 82.4 % inhibition at 40 mg/L.
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on results incl. tables'.
- Conclusions:
- EC50 (3 h): 44 mg/L
- Executive summary:
The test item showed 100 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/L. The EC50 was determined to be 44 mg/L.
Reference
Validity criteria for the measurement of the toxicity to microorganisms:
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
In a 2004 international ring test organized by ISO using activated sludge derived from domestic sewage, the EC50 of 3,5-dichlorphenol was found to lie in the range 2 mg/L to 25 mg/L for total respiration, 5 mg/L to 40 mg/L for heterotrophic respiration and 0.1 mg/L to 10 mg/L for nitrification respiration. If the EC50 of 3,5-DCP does not lie in the expected range, the test should be repeated with activated sludge from another source. | The EC 50 of the reference substance 3.5-dichlorophenol is in the range 5 - 30 mg/L, which is 5 mg/L above the range specified in the guideline. Considering the small deviation, this criterion is considered to be met. | Yes |
The test should be performed at a temperature within the range 20 ± 2°C. | The test temperature was 20 ± 2°C. | Yes |
The EC50 and, if possible, 95 % confidence limits, possibly EC20, EC80; possibly NOEC and the used statistical methods, if the EC50 cannot be determined shall be reported. | The EC50 including the 95% confidence limits are stated. The EC values were determined by probit analysis. | Yes |
Description of key information
The test item showed 100 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/L. The EC50 was determined to be 44 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 44 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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