[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From November 7 to November 17, 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

No analytical dose verification

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

(no analytical verification of test conc was done)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Reaction products of C14-C18 (even numbered) and C14-C18-unsatd. (even numbered) fatty acids or triglycerides with diethanolamine

Molecular formula:

not applicable gor UVCB

IUPAC Name:

Reaction products of C14-C18 (even numbered) and C14-C18-unsatd. (even numbered) fatty acids or triglycerides with diethanolamine

Test material form:

liquid: viscous

Details on test material:

see Section 1.2 for detailed composition

Reference

Constituent 1

Reference substance name:

Amides, C8-18 and C18-unsatd., N,N-bis(hydroxyethyl)

EC Number:

268-935-9

EC Name:

Amides, C8-18 and C18-unsatd., N,N-bis(hydroxyethyl)

Cas Number:

68603-42-9

IUPAC Name:

268-935-9

Test material form:

liquid

Details on test material:

- Name of test material (as cited in study report): Ufanon KDS (Coconut fatty acid diethanolamide, CAS 68603-42-9)
- Stability under test conditions: Soluble up to 1000 mg/L

Specific details on test material used for the study:

The discrepancy between originally usded identifier of the substance and new test material information is due to change in the main identifier through the Substance identity adaptation service offered by ECHA. The new identifier is covered by old identifier mentioned in Additional test material information section

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

The test solution was prepared in dilution water. The test material was found to be soluble up to 1000 mg/L in water.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Not specified
- Source: Toxicon's Daphnia culture
- Age at study initiation: Newly hatched, 6 to 24 h old
- Weight at study initiation: Not specified
- Length at study initiation: Not specified

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

21±1 °C

pH:

7.8±0.2

Dissolved oxygen:

Not specified, initial oxygen saturation > 90%

Salinity:

250±25 mg CaCO3/L

Nominal and measured concentrations:

Nominal: 0, 1, 2, 4, 8 and 16 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: Glass petri dishes with 50 mL solution
- Aeration: The dilution water was initially aerated to an oxygen saturation value of > 90%
- Renewal rate of test solution: Not applicable, static test
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): Two
- No. of vessels per control (replicates): Two


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reverse osmosis water

Composition of dilution water: According to ISO test water composition



OTHER TEST CONDITIONS
- Adjustment of pH: When required, it was done with HCl or NaOH
- Photoperiod: 16 h/8 h light: dark
- Light intensity: Subdued, no oher details


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilisation in the control and test animals at 24 and 48 h


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Control dish: Less than 10% immobility was observed in the control at 24 and 48 h.
Test dish: At 16 and 8 mg/L, 100 % immobilisation was observed.
Oxygen saturation: > 97 % oxygen saturation was maintained at the end 48 h. The pH was maintained around 8.0.
For details please refer to the attached results table in the 'background material' section.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the study, the 48 h EC50 was determined to be 3.2 mg/L (nominal) and the 48 h NOEC was 1.0 mg/L (nominal).

Executive summary:

A study was conducted to determine the acute toxicity of the test substance to Daphnia magna according to OECD Guideline 202, in compliance with GLP. The daphnids were exposed to nominal concentrations of 0, 1, 2, 4, 8 and 16 mg/L for 48 h under static conditions. Observations were performed at 0, 24 and 48 h to determine the % immobilised daphnids in the control and test groups. No analytical dose verification was conducted. Under the conditions of the study, the 48 h EC50 was determined to be 3.2 mg/L (nominal) and the 48 h NOEC was 1.0 mg/L (nominal) (Olsson, 1994).