Tris-(2-hydroxy-4-heteropolycycle-carboxylato-O´O) salt

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002-10-22 till 2002-11-08

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

, 1984

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

,signed 1999-05-25

Analytical monitoring:

yes

Details on sampling:

- Concentrations: The filtered test solution wer dissolved for measurement to obtain concentrations in the range of the calibration (20-80 mg/L)

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
A saturated solution of the test substance in dilution water was prepared by weighing 1000 mg/L test substance in dilution water and by shaking for 24 hours followed by membrane filtration (0.45 µm). The test solutions were prepared from stock solution by dilution. The concentrations of the test solutions were determined analytically at 0h and 48 h. Since not enough test animals were available, only two concentrations were used (dilution 1/10 and 2/10 and control).

In a second part 4 concentrations were tested (solution were prepared as described above): saturated solution, 4/10, 6/10, 8/10 and control

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS Berlin
- Source: UBA Berlin
- Age: 0-24 h
- Feeding during test: no

ACCLIMATION
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Tetra Animin(R)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20°C +- 2°C

pH:

7.8 +-0.2

Dissolved oxygen:

7.9 mg/L

Nominal and measured concentrations:

concentrations: control, 1/10, 2/10, 3/10, 4/10, 6/10, 8/10, saturated solution
measured 0h: 0, 68, 134, 267, 396, 523, 675 mg/L
measured 48h: 0, 72, 142, 271, 417, 580, 709 mg/L
recovery rates between 101 and 111 %
for calculation mean values were used as follows': 0, 70, 138, 269, 406, 551, 692 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 vessels à 20 mL test solution and 5 daphnids
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium according to ELEND

RANGE-FINDING STUDY
as range finding study the study results of "s_Rudolf_2002b" were used.

Reference substance (positive control):

yes

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

170 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Remarks on result:

other: 95%-CI 140-200 mg/L

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

< 70 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

551 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Results with reference substance (positive control):

Potassium dichromate
EC50 (24h): 1.4 mg/L

Reported statistics and error estimates:

program OriginTM

Validity criteria fulfilled:

yes

Conclusions:

The effect of the test item on Daphnia magna (age: < 24 h) was assessed in a 48 h acute toxicity test conducted in Elend-M4 freshwater, following OECD 202 (1984). 6 concentration (between 70 and 692 mg/L) were tested resulting in an EC50 (48h) of 170 mg/L (mean measured value, dissolved test item).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

170 mg/L

Additional information

The toxic effect of the test item to Daphnia magna (age: < 24h) was examined according to OECD 202 (1984) under static freshwater conditions over a period of 48 hours. In the definitive test, each test concentration and the control was tested with 4 replicated with five daphnids each. Effect concentrations were calculated based on mean measured dissolved test item concentration although analytical recoveries were in the range of 80 to 120 % throughout the test. After 48 hours of exposure the test item was found to immobilise Daphnia magna at concentrations < 70 mg/L (NOEC). The corresponding 48-hour EC50 value was calculated with 170 mg/L (95 %-CI: 140 -200 mg/L).