Registration Dossier
Registration Dossier
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EC number: 424-460-0 | CAS number: 283167-56-6
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 424-460-0
- EC Name:
- -
- Cas Number:
- 283167-56-6
- Molecular formula:
- C7 H10 N2 x 0.77 H3 P O4
- IUPAC Name:
- N1-methylbenzene-1,2-diamine; phosphoric acid
Constituent 1
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Study design
- Total exposure duration:
- 72 h
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 3 - <= 8.6 mg/L
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- >= 0.34 - <= 1.2 mg/L
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.68 mg/L
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of the present study with Selenastrum capricornutum, BIBR 277-MEPDA-P degraded rather rapidly during the 72-hour test
period. This degradation coincided with a change of the color of the test solutions, i.e. the intensity of the yellowish color of the solutions increased
during the test period. This increase of the color of the test solutions contributed to the effect on algal growth only at higher concentrations,
i.e. concentrations of 10 mg/l or higher. At 10wer concentrations the indirect effect of the color of the test solutions did not significantly contribute
to the effect on algal growth.
BIBR 277-MEPDA-P inhibited cell growth and reduced growth rate of the fresh water algae species Selenastrum capricornutum significantly at nominal
concentrations of 1.0 mg/l and higher.
The EC50 for cell growth inhibition (ESC50:0-72h) was 1.7 mg/l with a 95 % confidence interval ranging from 1.5 to 2.1 mg/l. The EC10 for cell
growth inhibition (ESCI0:0-72h) was 0.79 mg/l with a 95 % confidence interval ranging from 0.62 to 0.92 mg/l.
The EC50 for growth rate reduction decreased as exposure progressed and the final ERC50:0-72h was 4.5 mg/l with a 95 % confidence interval
ranging from 3.0 to 8.6 mg/l.
The ECI0 for growth rate reduction (ERC10:0-72h) was 0.78 mg/l with a 95 % confidence interval ranging from 0.34 to 1.2 mg/l.
The NOEC was 0.68 mg/l for both cell growth inhibition and growth rate reduction.
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