Dimethyl 2-nitroterephthalate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed and documented OECD study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl 2-nitroterephthalate

EC Number:

226-134-1

EC Name:

Dimethyl 2-nitroterephthalate

Cas Number:

5292-45-5

Molecular formula:

C10H9NO6

IUPAC Name:

dimethyl 2-nitroterephthalate

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Strain: Danio rerio (Hamilton-Buchanan)
- Source:Bio International B.V., Postbus 63, NL-5845 ZH St. Anthonis, The Netherlands
- Age at study initiation : about 12 weeks
- Length at study initiation: 3.0 - 3.3 cm (average 3.1 cm)
- Feeding during test: twice daily ad libitum
- Food type: Tetra Min, tetra Werke, Melle (Germany)

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test
- Type and amount of food: Tetra Min, tetra Werke, Melle (Germany)
- Feeding frequency: twice daily ad libitum

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

no

Test conditions

Test temperature:

21.1 - 21.9 °C

pH:

7.4 - 8.2

Dissolved oxygen:

6.6 - 9.7 mg/L

Nominal and measured concentrations:

nominal mg/L 0.5 1.0 2.2 5.0 10
measured (24h) mg/L 0.12 0.27 0.76 1.78 4.27

Details on test conditions:

TEST SYSTEM
- Test vessel: glass, 20 x 15 x 20 cm , open
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water acc. ISO/DIN 7346/1
OTHER TEST CONDITIONS
- Photoperiod: illumination from 6:00 am to 6:00 pm
- Light intensity: about 700 lux

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

7.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

1.7 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

2.7 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 6

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

4 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

Probit analysis could not be carried out due to the steep dose response curve. As the factor for the concentration steps was 2.2 (third root of 10), no further concentrations were tested. After 24 and 48 hours the LC50 range is given. After 72 and 96 hours the LC 50 is specified as the geometric mean of the LC0 and LC 100

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this test the LC50 of dimethy-2-nitroterephthalate is 7.1 mg/L (based on nominal concentrations) rsp. 2.7 mg/L (based on measured concentrations.)

Executive summary:

The 96 -hour acute toxicity study dimethy-2-nitroterephthalate in zebrafish (Danio rerio) was conducted in a semistatic system with renewal of the test solution in 24 hours interval, because in a static system the test substance was not analytically detectable in a sufficient degree for 96 hours. The nominal concentrations tested were 0.5, 1.0, 2.2, 5., 10.0 mg/L and a control (0 mg/L).

Symptoms and mortality (7/7) werde observed at 10 mg/L. The 1 - 5 mg/L groups exhibited symptoms but no mortality. The 0.5 mg/L group showed no effect in comparison with the control. In the control group no mrtality was observed.

Under the conditions of this test the LC50 of dimethy-2-nitroterephthalate is 7.1 mg/L (based on nominal concentrations) rsp. 2.7 mg/L (based on measured concentrations.)