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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Description of key information

No effects up to the limit of water solubility (WS < 0.05 mg/L).

Key value for chemical safety assessment

Additional information

A study evaluating the acute toxicity of 1,2,3-propanetriyl trioleate (CAS No. 122-32-7) to fish is available (Bogers, 1988). This test was conducted according to the German Method Evaluation of hazardous substances in water- Determination of the acute toxicity to Fish, under GLP conditions. Fish (Cyprinus carpio) were exposed to the test substance within a static water regime at 23 ± 2°C. After the exposure period, no mortality was observed in treatment or control groups. The LC50 (48 h) was determined to be > 1000 mg/L (nominal concentration).

According to the Guidance on information requirements and chemical safety assessment, chapter R.7B, results obtained at shorter test durations that the standard 96 hours (OECD 203) should be treated with caution and always in combination with other data (read-across, non-testing, etc.). Therefore, a Weight of Evidence (WoE) approach is applied to cover adequately this endpoint, using the available data from 1,2,3-propanetriyl trioleate in conjunction with an additional acute fish test from a structurally related analogue substance (Glycerides, C14-18 and C16-18 unsatd. mono-, di- and tri-, CAS No. 91052-28-7) in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5. Both are UVCB substances, formed from the esterification of fatty acids and glycerol as alcohol component, having a high content (>60%) of C18 unsaturated fatty acid and to a certain extent C16, C14 and C12. Furthermore, their water solubilities are comparable (< 0.05 mg/L). Therefore, these two substances are expected to show similar behaviour in the water phase and comparable degrees of toxicity to aquatic organisms, being expected to be slightly higher for CAS No. 91052-28-7 due to its higher monoester content compared to the triester present for CAS No. 122-32-7 (less degree of esterification will reduce size, increasing the potential to cross biological membranes). Based on the above information, the use of data from Glycerides, C14-18 and C16-18 unsatd. mono-, di- and tri- as read-across for 1,2,3-propanetriyl trioleate is considered justified.


The study conducted with Glycerides, C14-18 and C16-18 unsatd. mono-, di- and tri- (CAS No. 91052-28-7) follows the German national Guideline DIN 38412/15 (Examination of water, waste water and sludge; bioassays (L); determination of the effect of substances in water on fish- Fish test (L15)). In this static test, fish (Leuciscus idus) were exposed to the test substance for 48 hours. After this period, no mortality or any other effects were reported at any of the concentrations tested (LC50 (48 h) > 10000 mg/L).


The results from the two 48h-studies in combination provide enough evidence to state that 1,2,3-propanetriyl trioleate is not expected to be acutely toxic to fish up to the limit of its water solubility.