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EC number: 222-852-4 | CAS number: 3634-83-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- September 9, 1981 - December 3, 1981
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Conducted equivalent to OECD 401. The dosing volume is not according to this Guideline; no purity information provided; non-GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- yes
- Remarks:
- ; dosing volume: not constant at all dose levels and up to 25 mL/kg has been dosed (Guideline: max. 10 ml/kg)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 1,3-bis(isocyanatomethyl)benzene
- EC Number:
- 222-852-4
- EC Name:
- 1,3-bis(isocyanatomethyl)benzene
- Cas Number:
- 3634-83-1
- Molecular formula:
- C10H8N2O2
- IUPAC Name:
- 1,3-bis(isocyanatomethyl)benzene
- Details on test material:
- - Name of test material (as cited in study report): XDI (Takemate 500)
- Analytical purity: no data
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: CD (Sprague-Dawley derived)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River U.K. Limited, Margate, Kent, England
- Age at study initiation: approximately 4 to 6 weeks of age
- Weight at study initiation: 95 to 130 g
- Fasting period before study: approximately 18 +/- 2 hours prior to dosing
- Housing: metal cages with wire mesh floors (randomly allocated per dose group)
- Diet (e.g. ad libitum): ad libitum (standard laboratory diet)
- Water (e.g. ad libitum): ad libitum
- Acclimation period: minimum period of 6 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22.5 +/- 2.5 degrees Celsius
- Humidity (%): not controlled but remained within a range of 31-57%
- Air changes (per hr): approximately 15 per hr
- Photoperiod (hrs dark / hrs light): 12/12 hrs
IN-LIFE DATES: no data
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 20% w/v
- Amount of vehicle (if gavage): varied, depending on the dose
MAXIMUM DOSE VOLUME APPLIED: 25 mL/kg bw - Doses:
- Preliminary study: 0.5; 2.0; 5.0 and 8.0 g/kg bw
Main study: 0; 2.5; 3.2; 4.0; 5.0 g/kg bw - No. of animals per sex per dose:
- Preliminary study: 2 males + 2 females per dose
Main study: 5 males + 5 females per dose - Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
Day 1: Animals were observed soon after dosing; then at frequent intervals for the remainder of Day 1.
Day 2-14: On subsequent days the animals were observed at least once. Clinical signs were recorded at each observation.
During the observation period the following were recorded:
-Approximate time of death
-The nature, severity, approximate time of onset and duration of each toxic sign
-Individual bodyweights on days: 1, 8 and 15
- Necropsy of survivors performed: yes - Statistics:
- The acute oral median LD50 to rats was calculated using the method of Finney (1971) Probit Analysis (3rd edition) Cambridge University Press
Results and discussion
- Preliminary study:
- See the table mentioned under the section "Any other information on results inc. tables" for the results of the preliminary study.
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 3 200 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 2 500 - 3 900
- Mortality:
- See the tables in the section "Any other information on results incl. tables"
- Clinical signs:
- other: See the table in the section "Any other information on results incl. tables"
- Gross pathology:
- Autopsy of died rats revealed congestion or haemorrhage of the lungs, pallor of the liver, spleen and kidneys and in rats dying overnight, an apparent brittleness of the stomage. These findings were accompanied by congestion of the blood vessels of the stomach in one male and two females at 3.2 g/kg; one male at 4.0 g/kg and two males and one female at 5.0 g/kg. Gaseous contents of the stomach were observed in one male and one female at 3.2 g/kg, one male at 4.0 g/kg and one male and one female at 5.0 g/kg.
Survivors showed no abnormalities.
Any other information on results incl. tables
Results of the preliminary study:
Dosage (g/kg) |
Mortality ratio |
Time of death after dosing (hours) |
||
Male |
Female |
Combined |
||
0.5 |
1 / 2 |
0 / 2 |
1 / 4 |
<4 days |
2.0 |
0 / 2 |
0 / 2 |
0 / 4 |
- |
5.0 |
2 / 2 |
2 / 2 |
4 / 4 |
<22 - <70 |
8.0 |
2 / 2 |
2 / 2 |
4 / 4 |
<4 - <22 |
Results of the main study:
Sex |
Dosage (g/kg) |
Average bodyweight (g) at: |
Mortality ratio |
Time of death after dosing (hours) |
||
Dosing |
1 week |
2 weeks |
||||
Male |
0 |
112 |
193 |
255 |
0 / 5 |
- |
2.5 |
117 |
181 |
247 |
2 / 5 |
<70 |
|
3.2 |
122 |
181 |
248 |
2 / 5 |
<22 - <46 |
|
4.0 |
122 |
157 |
215 |
3 / 5 |
<22 - <46 |
|
5.0 |
125 |
171 |
235 |
4 / 5 |
<22 - <70 |
|
Female |
0 |
104 |
166 |
194 |
0 / 5 |
- |
2.5 |
107 |
154 |
186 |
1 / 5 |
<46 |
|
3.2 |
103 |
146 |
184 |
2 / 5 |
<22 - <27 |
|
4.0 |
106 |
138 |
175 |
4 / 5 |
<22 - <70 |
|
5.0 |
105 |
- |
- |
5 / 5 |
<22 - <46 |
Recovery of the survivors, as judged by external appearance and behaviour, was apparently complete by Day 10. Clinical signs:
Signs |
Signs of reaction ratio |
||||
Dosage (g/kg) |
|||||
0 |
2.5 |
3.2 |
4.0 |
5.0 |
|
Pilo-erection |
10 / 10 |
10 / 10 |
10 / 10 |
10 / 10 |
10 / 10 |
Abnormal body carriage (hunched posture) |
0 / 10 |
10 / 10 |
10 / 10 |
10 / 10 |
10 / 10 |
Abnormal gait (waddling) |
0 / 10 |
10 / 10 |
10 / 10 |
10 / 10 |
10 / 10 |
Lethargy |
0 / 10 |
10 / 10 |
10 / 10 |
10 / 10 |
10 / 10 |
Decreased respiratory rate |
0 / 10 |
10 / 10 |
10 / 10 |
10 / 10 |
10 / 10 |
Diarrhoea |
7 / 10 |
10 / 10 |
10 / 10 |
10 / 10 |
10 / 10 |
Pallor of extremities |
0 / 10 |
10 / 10 |
10 / 10 |
10 / 10 |
10 / 10 |
Ptosis |
0 / 10 |
6 / 10 |
9 / 10 |
10 / 10 |
10 / 10 |
Diuresis |
0 / 10 |
2 / 10 |
2 / 10 |
2 / 10 |
0 / 10 |
Increased salivation |
0 / 10 |
5 / 10 |
6 / 10 |
10 / 10 |
10 / 10 |
Gasping respiration |
0 / 10 |
2 / 10 |
1 / 10 |
1 / 10 |
2 / 10 |
Râles |
0 / 10 |
5 / 10 |
2 / 10 |
6 / 10 |
5 / 10 |
Abnormal gait (walking on toes) |
0 / 10 |
0 / 10 |
0 / 10 |
2 / 10 |
1 / 10 |
Increased lacrimation |
0 / 10 |
3 / 10 |
6 / 10 |
7 / 10 |
5 / 10 |
Bloodstained muzzle |
0 / 10 |
2 / 10 |
2 / 10 |
4 / 10 |
3 / 10 |
Body tremors |
0 / 10 |
0 / 10 |
4 / 10 |
2 / 10 |
4 / 10 |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- In a study conducted similar to OECD 401, the LD50 oral in rats was shown to be 3200 mg/kg (95% confidence limits: 2500 to 3900 mg/kg).
- Executive summary:
The acute oral toxicity in rats has been studied similar to OECD 401. The only deviation from the OECD Guideline were the used dosing volumes, which were not constant at all dose levels and up to 25 mL/kg (Guideline: max. 10 ml/kg). The substance was dosed in corn oil at 0, 2.5, 3.2, 4.0 and 5.0 g/kg bw in 5 male and 5 female rats (doses based on the results from the preliminary study). Signs of reaction to treatment observed shortly after dosing in all treated rats included pilo-erection, an abnormal body carriage (hunched posture), abnormal gait (waddling), lethargy, decreased respiratory rate, pallor of the extremities and diarrhoea. Mortalities occurred amongst rats treated at 2.5 g/kg and above from within 22 hours to 70 hours of dosing. Autopsy of died rats revealed congestion or haemorrhage of the lungs, pallor of the liver, spleen and kidneys and in rats dying overnight, an apparent brittleness of the stomage.
Terminal autopsy findings were within normal limits. Depressed bodyweight gains were recorded in all surviving treated rats during the first week of observation only, compared to the control. The acute oral toxicity was determined to be 3200 mg/kg (95% confidence limits: 2500 to 3900 mg/kg). Based on this result, the substance does not need to be classified for acute toxicity by the oral route.
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