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EC number: 231-659-4 | CAS number: 7681-11-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental result performed using standard methods of analysis
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- As no mortalities were found in 96 hrs, the experiment was continued up to seven days and the no observed effect concentration (NOEC) was noted.
- Principles of method if other than guideline:
- Short term toxicity study of test chemical were carried out on the Danio rerio (previous name: Brachydanio rerio). Test conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test).
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring test water. Deionized water was used
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra Fish
- Age at study initiation: 3-4 months
- Length at study initiation (mean): 2.3 cm
- Weight at study initiation (mean): 0.203 g
- Feeding during test: Yes
- Food type: Standard brand feed
- Frequency: once daily
- Housing: The fishes were kept in a static tank in tap water, under natural conditions along with proper feed and aeration.
- Duration of housing :15 days
- Photoperiod:12 hours light and 12 hours darkness
- Oxygen content:7.4
- pH: Between 6-7
- Water temperature: 29°C
- Mortality (%): 0% - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- As no effects were observed after 96 hrs, test extended for 7 days.
- Test temperature:
- 29°C
- pH:
- 6-7
- Dissolved oxygen:
- 6-7.9 mg/L
- Nominal and measured concentrations:
- 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass bowl aquaria
- Material, size, headspace, fill volume: 12” glass bowl aquaria were used
- Aeration: Aeration was provided 1 hr before the starting of experiment and also during the experiment when requiredWithdrawal of food: 1 day before and during exposure
- No. of organisms per vessel: 8 fishes - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: No effects were observed
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Other details not known
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Other details not known
- Sublethal observations / clinical signs:
Mortality and symptoms
Nominal conc. (mg/l)
Number of fish
Mortality (cumulated) after
1stDay
2ndDay
3rdDay
4thDay
5th Day
6thDay
7thDay
0 (control)
8
0
0
0
0
0
0
0
100
8
0
0
0
0
0
0
0
Nominal conc. (mg/l)
Number of fish
Symptoms after
1stDay
2ndDay
3rdDay
4thDay
5th Day
6thDay
7thDay
0 (control)
8
NS8
NS8
NS8
NS8
NS8
NS8
NS8
100
8
NS8
NS8
NS8
NS8
NS8
NS8
NS8
Explanation of symptoms:
A: apathy
N: narcotic-like state
C: swimming near water surface
D: swimming at the bottom of bowl
M: Slow movements compared to control
NS: No abnormal symptoms
Number behind symbol for symptom= number of affected fish
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the mortality of Danio rerio (previous name: Brachydanio rerio) by the test chemical after 96 hrs of exposure, LC50 was observed to be > 100 mg/l. As no effect were observed at 100 mg/l test extended for 7 days. After 7 days of exposure no effect were observed.
- Executive summary:
Short term toxicity study of test chemical were carried out on the Danio rerio (previous name: Brachydanio rerio). Test conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test). The limit test at 100 mg/L was conducted. The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring test water. Deionized water was used. Observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. The test vessels used were all glass bowl aquaria of 12” each. 3-4 months old 8 fishes in each vessel were added. In a 96 h study for test chemical on Danio rerio, at a test concentration of 100 mg/L, the fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l. No mortalities were observed in the control aquaria. Based on the mortality of Danio rerio (previous name: Brachydanio rerio) by the test chemical after 96 hrs of exposure, LC50 was observed to be > 100 mg/l. As no effect were observed at 100 mg/l test extended for 7 days. After 7 days of exposure no effect were observed. Thus on the basis of LC50 > 100 mg/l, chemical consider to be nontoxic and can be consider to be not classified as per the CLP classification criteria.
Reference
Description of key information
Short term toxicity study of test chemical were carried out on the Danio rerio (previous name: Brachydanio rerio). Test conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test). The limit test at 100 mg/L was conducted. The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring test water. Deionized water was used. Observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. The test vessels used were all glass bowl aquaria of 12” each. 3-4 months old 8 fishes in each vessel were added. In a 96 h study for test chemical onDanio rerio, at a test concentration of 100 mg/L, the fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l. No mortalities were observed in the control aquaria. Based on the mortality of Danio rerio (previous name: Brachydanio rerio) by the test chemical after 96 hrs of exposure, LC50 was observed to be > 100 mg/l. As no effect were observed at 100 mg/l test extended for 7 days. After 7 days of exposure no effect were observed. Thus on the basis of LC50 > 100 mg/l, chemical consider to be nontoxic and can be consider to be not classified as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Data available for the test chemicals including structurally and functionally similar read across chemicals, study has been reviewed to determine the short term toxicity on fish of the test chemical. The studies are as mentioned below:
In the first study short term toxicity of test chemical were carried out on the Danio rerio (previous name: Brachydanio rerio). Test conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test). The limit test at 100 mg/L was conducted. The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring test water. Deionized water was used. Observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. The test vessels used were all glass bowl aquaria of 12” each. 3-4 months old 8 fishes in each vessel were added. In a 96 h study for test chemical on Danio rerio, at a test concentration of 100 mg/L, the fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l. No mortalities were observed in the control aquaria. Based on the mortality of Danio rerio (previous name: Brachydanio rerio) by the test chemical after 96 hrs of exposure, LC50 was observed to be > 100 mg/l. As no effect were observed at 100 mg/l test extended for 7 days. After 7 days of exposure no effect were observed. Thus on the basis of LC50 > 100 mg/l, chemical consider to be nontoxic and can be consider to be not classified as per the CLP classification criteria.
Above study was supported by the second study. Short term toxicity of test chemical were determine on the growth of fishes Oncorhynchus mykiss (previous name: Salmo gairdneri). Test conducted under the static system for 96 hrs. The lethal concentration (LC50) value of test chemical on fish (Oncorhynchus mykiss) in a 96 hrs study on mortality effect was determine to be 2190 mg/L. Thus considering CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit short term toxicity to fish (Oncorhynchus mykiss).
In the third study from secondary source short term toxicity of test chemical were determine on the growth of fish Oncorhynchus mykiss (previous name: Salmo gairdneri). Test conducted under the static system on 50 MM Oncorhynchus mykiss. 96 hrs of incubation were provided to the fishes. After the exposure of test chemical with Oncorhynchus mykiss (previous name: Salmo gairdneri) for 96 hrs, LC50 was observed to be 896 mg/l. Based on the LC50 value, chemical consider to be nontoxic and not classified as per the CLP classification criteria.
Based on the above studies, chemical concluded to be nontoxic and thus can be consider to be not classified as per the CLP classification criteria.
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