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Diss Factsheets
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EC number: 203-350-4 | CAS number: 105-99-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1988
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Only secondary literature. Original report not available.
- Principles of method if other than guideline:
- Oxygen consumption test (Huels method)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Chemical name of vehicle: Nonylphenol-10EO-5PO - Test organisms (species):
- Pseudomonas putida
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 5 h
- Nominal and measured concentrations:
- not stated
- Reference substance (positive control):
- not specified
- Duration:
- 5 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 630 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- not specified
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 - 13 Oct 1998
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP study similar to guideline study with acceptable restrictions: The test substance is tested using only one replicate for each test concentration. No analytics of the test substance is performed.
- Qualifier:
- according to guideline
- Guideline:
- other: Amtsblatt der EG L 133, Teil C: Biologische Abbaubarkeit: Prüfung der Atmungshemmung
- Deviations:
- yes
- Remarks:
- The incubation period was 3 h instead of 30 minutes under continuous aeration (this was recommended by the German federal Environmental Agency
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- because of the high oxygen demand of the activated sludge, a inoculum concentration of only 0.48 g/L were used
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was directly added to the test vessel and stirred overnight. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: Activated sludge collected from water treatment plant predominantly processing municipal waste water; Wupperverband, Germany; date of collection: 12 Oct 1998
- Pretreatment: none
- Initial biomass concentration: 480 mg/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.4 - 8.2
- Dissolved oxygen:
- 5.8 - 8.6 mg/L at test start
2.5 - 8.0 mg/L at test end - Nominal and measured concentrations:
- Nominal: 1000, 1800, 3200, 5600 and 10000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Aeration: continuously
- No. of vessels per concentration: 1
- No. of vessels per control: 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
- Intervals of water quality measurement: Oyxygen concentration was measured at the beginning and at the end of the test. Temperature and pH was measured once per test concentration after 6-8 minutes.
EFFECT PARAMETERS MEASURED: Inhibition of respiration was recorded after 3 h test duration. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorphenol
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 = 12 mg/L; EC10 = 2.9 mg/L
Referenceopen allclose all
Table 1: Test results
Nominal concentration [mg/L] |
Respiration rate [mg/L*h] |
Abiotic loss of oxygen [mg/L*h] |
Inhibition [%] |
1000 |
27.8 |
0.0* |
0.0 |
1800 |
27.4 |
0.0* |
0.0 |
3200 |
25.5 |
0.0* |
6.3 |
5600 |
29.1 |
0.0* |
0.0 |
10000 |
28.0 |
0.0 |
0.0 |
Control 1 |
27.0 |
|
|
Control 2 |
27.4 |
|
*: The abiotic oxygen loss was measured in the 10000 mg/L treatment. As no loss of oxygen was observed, it was assessed that also no loss occurred at the lower test concentrations.
Description of key information
EC10 (3h) > 10000 mg/L (nominal) for respiration rate of activated sludge (similar to OECD 209)
Key value for chemical safety assessment
Additional information
One study investigating the short-term toxicity to aquatic microorganisms of Dibutyl adipate (CAS 105-99-7) is available. The GLP study was performed similar to OECD 209 using activated sludge of a predominately domestic sewage as inoculum (Müller, 1998). The test substance was directly added to the vessels. The effect of the test substance on the respiration rate was determined under static conditions using nominal concentrations in the range of 1000 to 10,000 mg/L. No respiration inhibition was measured. Hence, the 3 h-EC10 is determined to be > 10,000 mg/L on the basis of the nominal test concentration.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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