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EC number: 700-579-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
- GLP compliance:
- no
Test material
- Reference substance name:
- tetrakis(4-{[4-(diethylamino)phenyl][4-(ethylamino)naphthalen-1-yl]methylidene}-N,N-diethylcyclohexa-2,5-dien-1-iminium) 2-(dodecan-5-yl)-4-(4-sulfonatophenoxy)benzene-1-sulfonate 2-(dodecan-5-yl)-4-[3-(dodecan-5-yl)-4-sulfonatophenoxy]benzene-1-sulfonate
- EC Number:
- 700-579-6
- Molecular formula:
- C90H114O7S2N6 (MW = 1454) and C102H138O7S2N6 (MW = 1622)
- IUPAC Name:
- tetrakis(4-{[4-(diethylamino)phenyl][4-(ethylamino)naphthalen-1-yl]methylidene}-N,N-diethylcyclohexa-2,5-dien-1-iminium) 2-(dodecan-5-yl)-4-(4-sulfonatophenoxy)benzene-1-sulfonate 2-(dodecan-5-yl)-4-[3-(dodecan-5-yl)-4-sulfonatophenoxy]benzene-1-sulfonate
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- - Batch number: 310551
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 14 ml of test item diluted in 1 L liters of ultra-pure water (stock solution).
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): not mentionned
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Age of inoculum (at test initiation): 3 days
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22°C +/2
- pH:
- 8.1 à 9.1
- Nominal and measured concentrations:
- Nominal concentration:
0-7.0-8.4-9.8-11.2-12.6 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer
- Material, size, headspace, fill volume: Erlenmeyer with 60 mL of the tested solution
- Initial cells density: 1000 cells/mL
- No. of vessels per concentration : 3
- No. of vessels per control (replicates): 6
OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Light intensity and quality:4500 lux
- stirring: 250 min-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Mallassez counting chamber
TEST CONCENTRATIONS
- the maximum tested concentration was 12,6 mg/L. According to the maximum water solubility (14 mg/l) and the quantification limit of the algal growth inhibition test: 90 % of the stock solution, the maximal tested concentration was 12.6 mg/L.
A stock solution at 14 mg/L was prepared with pure water. Then a feeding media was added so as to represent 10% of the final solution.
Therefore the highest concentration was 12,6 mg/L (0.9 x 14) - Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 12.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 12.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 12.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 12.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no mentionned
- the results correspond to the maximal concentration tested - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50 (72h): 1.21 mg/L (belongs to the recommended range of values: 0.92 - 1.46 mg/L) - Reported statistics and error estimates:
- CE x % calculated using the Hill equation (macro Regtox_ev6.6.2xls)
NOEC calculated with the T-test of Bonferroni (Toxcalc software)
Any other information on results incl. tables
Results at t = 24h, 48h and 72h:
t = 24 h |
||||||||
Concentration (mg/L) |
Cells Concentration x 106 |
|
|
|||||
1 |
2 |
3 |
4 |
5 |
6 |
Average Cells number |
Cells Inhibition |
|
0 |
0.075 |
0.073 |
0.076 |
0.079 |
0.077 |
0.079 |
0.08 |
|
7.0 |
0.071 |
0.082 |
0.079 |
|
|
|
0.077 |
-1% |
8.4 |
0.072 |
0.077 |
0.068 |
|
|
|
0.072 |
6% |
9.8 |
0.074 |
0.078 |
0.077 |
|
|
|
0.076 |
0% |
11.2 |
0.068 |
0.076 |
0.057 |
|
|
|
0.067 |
12% |
12.6 |
0.056 |
0.067 |
0.074 |
0.056 |
0.073 |
0.077 |
0.067 |
12% |
t = 24 h |
||||||||
Concentration (mg/L) |
Growth Rate |
|
|
|||||
1 |
2 |
3 |
4 |
5 |
6 |
Average Cells number |
Growth rate Inhibition |
|
0 |
2.01 |
1.99 |
2.03 |
2.07 |
2.05 |
2.07 |
2.04 |
|
7.0 |
1.96 |
2.11 |
2.06 |
|
|
|
2.04 |
0% |
8.4 |
1.97 |
2.04 |
1.91 |
|
|
|
1.97 |
3% |
9.8 |
2.00 |
2.06 |
2.04 |
|
|
|
2.03 |
0% |
11.2 |
1.92 |
2.03 |
1.74 |
|
|
|
1.90 |
7% |
12.6 |
1.72 |
1.90 |
2.00 |
1.73 |
1.99 |
2.05 |
1.90 |
7% |
t = 48 h |
||||||||
Concentration (mg/L) |
Cells Concentration x 106 |
|
|
|||||
1 |
2 |
3 |
4 |
5 |
6 |
Average Cells number |
Cells Inhibition |
|
0 |
0.57 |
0.64 |
0.64 |
0.71 |
0.65 |
0.62 |
0.64 |
|
7.0 |
0.54 |
0.65 |
0.58 |
|
|
|
0.59 |
7% |
8.4 |
0.63 |
0.77 |
0.56 |
|
|
|
0.65 |
-2% |
9.8 |
0.60 |
0.59 |
0.62 |
|
|
|
0.60 |
5% |
11.2 |
0.431 |
0.477 |
0.587 |
|
|
|
0.50 |
22% |
12.6 |
0.55 |
0.44 |
0.37 |
0.52 |
0.55 |
0.60 |
0.50 |
21% |
t = 48 h |
||||||||
Concentration (mg/L) |
Growth Rate |
|
|
|||||
1 |
2 |
3 |
4 |
5 |
6 |
Average Cells number |
Growth rate Inhibition |
|
0 |
2.02 |
2.08 |
2.08 |
2.13 |
2.09 |
2.06 |
2.08 |
|
7.0 |
2.00 |
2.09 |
2.03 |
|
|
|
2.04 |
2% |
8.4 |
2.07 |
2.17 |
2.01 |
|
|
|
2.08 |
0% |
9.8 |
2.05 |
2.04 |
2.06 |
|
|
|
2.05 |
1% |
11.2 |
1.88 |
1.93 |
2.04 |
|
|
|
1.95 |
6% |
12.6 |
2.01 |
1.89 |
1.80 |
1.98 |
2.00 |
2.05 |
1.95 |
6% |
t = 72 h |
||||||||
Concentration (mg/L) |
Cells Concentration x 106 |
|
|
|||||
1 |
2 |
3 |
4 |
5 |
6 |
Average Cells Number |
Cells Inhibition |
|
0 |
3.54 |
3.70 |
4.12 |
3.83 |
3.61 |
3.60 |
3.73 |
|
7.0 |
3.77 |
3.95 |
3.85 |
|
|
|
3.86 |
-3% |
8.4 |
4.02 |
4.25 |
3.97 |
|
|
|
4.08 |
-9% |
9.8 |
3.25 |
3.20 |
3.89 |
|
|
|
3.45 |
8% |
11.2 |
3.54 |
3.82 |
4.02 |
|
|
|
3.79 |
-2% |
12.6 |
3.75 |
3.39 |
3.22 |
4.36 |
3.76 |
3.84 |
3.72 |
0% |
t = 72 h |
|
|
||||||||
Concentration (mg/L) |
Growth Rate |
|
|
|
|
|||||
1 |
2 |
3 |
4 |
5 |
6 |
Average Cells number |
Growth rate Inhibition |
Standard deviation |
Variation coefficient (%) |
|
0 |
1.96 |
1.97 |
2.01 |
1.98 |
1.96 |
1.96 |
1.97 |
|
0.017 |
0.86 |
7.0 |
1.98 |
1.99 |
1.98 |
|
|
|
1.98 |
-1% |
0.006 |
0.31 |
8.4 |
2.00 |
2.02 |
2.00 |
|
|
|
2.00 |
-2% |
0.010 |
0.49 |
9.8 |
1.93 |
1.92 |
1.99 |
|
|
|
1.95 |
1% |
0.029 |
1.51 |
11.2 |
1.96 |
1.98 |
2.00 |
|
|
|
1.98 |
0% |
0.017 |
0.88 |
12.6 |
1.98 |
1.94 |
1.92 |
2.03 |
1.98 |
1.98 |
1.97 |
0% |
0.032 |
1.63 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the experimental conditions:
CE 50r - 72h > 12.6 mg/L
NOEC - 72 h > 12.6 mg/L - Executive summary:
The acute algal toxicity test for the substance Sepisol Fast Blue 85219 was carried out to determine the ecotoxicological profile of the test substance against an unicellular green algae. As test organism Pseudokirchnerella subcapitata was used and the NF EN ISO 8692 (2005) standard method was followed.
The parameters which were to be determined were the effective concentrations (EC) and the no observed effect concentration 72h (NOEC-72h) values with regard to the growth rate (ErC).
The following nominal concentrations were tested: 0; 7.0; 8,4; 9,8; 11,2 and 12,6 mg/L. The maximum tested concentration was 12,6 mg/L. According to the maximum water solubility and the quantification limit of the algal growth inhibition test: 90 % of the stock solution, the maximal tested concentration was determined to be 12.6 mg/L.
A stock solution was prepared by diluting 14 mg of test material in 1 liter of pure water.
Each concentration and the controls were inoculated with approximately 10 000 algae per mL.
The test was carried out over 72 hours at a temperature of 22°C +/- 2 and constantly stirred (250 min-1). Each vessel concentration was tested 3 times. 5 controls were run with the same experimental conditions. Algal concentration was counted using a Mallassez counting chamber. Once a day the extinction of an aliquot of the test-vessels (incubation) was measured.
Validation experiments and the precultivation ensured that the algal were of appropriate vialibilty and susceptibility.
The results of this study showed, that the substance Sepisol Fast Blue 85219 did not inhibit cells nor reduce the growth rate of the test organism at 12, 6 mg/L. The quantification limit of the algal growth inhibition test being 90 % of the stock solution, the maximal tested concentration was 12.6 mg/L.
The ErC 10 (72h) and the NOEC (72h) were thus determined to be higher than 12.6 mg/L (with 12.6 being the quantification limit of the inhibition test: 90 % and the maximum water solubity being 14 mg/L).
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