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EC number: 480-420-2 | CAS number: 144702-27-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2 August- 3 August 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted in GLP compliance and in accordance with several internationally established guidelines (OECD, EEC, EPA guidelines, see below).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 480-420-2
- EC Name:
- -
- Cas Number:
- 144702-27-2
- Molecular formula:
- C33 H29 N5
- IUPAC Name:
- 4'-{[4-methyl-6-(1-methyl-1H-1,3-benzodiazol-2-yl)-2-propyl-1H-1,3-benzodiazol-1-yl]methyl}-[1,1'-biphenyl]-2-carbonitrile
- Test material form:
- other: solid
- Details on test material:
- - Name of test material (as cited in study report): BIBR 277 Nitril
- Physical state: Solid
- Analytical purity: 99.7%
- Lot/batch No.: T02/03
- Expiration date of the lot/batch: April 2006
- Storage condition of test material: At room temperature, dark and dry.
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): STP for domestic sewage in Veszprem, Hungary
The activated sludge was washed by centrifugation, supernatant liquid was decanted. The solid material was re-suspended in an isotonic saline solution and centrifuged again (procedure was repeated twice). An aliquot of the final sludge suspension was weighed, dried and the ratio of wet/dry weight was determined.
based on this ratio, calculated aliquots of washed sludge suspension containing 4g dry material/L were mixed with test water and aerated until use. Before use, sludge was filtered with cotton wool. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 2 mg/L
- Based on:
- ThOD/L
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: grade salts were added to deionised water to give stock solutions. Stock solutions were diluted in deionised water, aerated for 20 minutes and allowed to stand for 20 hrs at the test temperature. Dissolved oxygen concentration was about 9.0 mg/L prior to starting the test.
- Test temperature: 20°C
- pH: 7.2 (at the start of the test)
- pH adjusted: no
- Aeration of dilution water: yes (see above)
TEST SYSTEM
- Culturing apparatus: BOD 300 mL bottles
- Number of culture flasks/concentration: Each 16 (+2 reserve) bottles containing: a) the test item and inoculum, b) the reference item and inoculum (positive control), c) only inoculum (inoculum control) and d) test item, reference item and inoculum (toxicity control).
- Method used to create aerobic conditions: inoculum and medium solutions were sufficiently aerated prior to the start of the test.
- Measuring equipment: self-stirring O2 electrode
- Test performed in closed vessels: yes
- Details of trap for CO2 and volatile organics if used: not used
SAMPLING
- Sampling frequency: oxygen was measured at days 0, 2, 5, 7, 12, 14, 21 and 28 after the start of the test.
- Sampling method: no sampling was performed for oxygen analysis, the oxygen concentrations were measured with an oxygen meter with a stirring O2 electrode.
- Sterility check if applicable: no
- Sample storage before analysis: no
- Other: Nitrite and nitrate concentrations were measured (representing oxidized nitrogen within the test solutions) via HPLC-UV analysis. Samples were directly injected.
CONTROL AND BLANK SYST EM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes
- Other: see above for details
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1
- Sampling time:
- 28 d
- Details on results:
- The residual oxygen concentration did not drop below 0.5 mg O2/L at any time. The difference in duplicate samples was less than 20%.
BOD5 / COD results
BOD5 / COD
- Parameter:
- BOD5
- Value:
- mg O2/g test mat.
- Results with reference substance:
- The percentage of degradation of the reference substance item reached the passt level for ready biodegradability (> 60%) within 14 days.
The oxygen consumption in the inoculum control did not exceed 1.5 mg/L after 28 days.
Any other information on results incl. tables
The BOD valued were not corrected for nitrification, because the quantity of total oxidised nitrogen (nitrite and nitrate) were below the LOQ of HPLC-UV analysis or was about 0 after the correction with control values.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- Under the test conditions the percentage biodegradation of BIBR 277 Nitril reached a mean of -1% after 28 days of incubation, based on ThOD (No3).The test item is regarded to be not readily biodegradable.
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