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Diss Factsheets
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EC number: 204-496-1 | CAS number: 121-73-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Endpoint summary
Administrative data
Description of key information
No reactions were observed in any of the cases so that m-chloro-nitrobenzene therefore it was evaluated as nonsensitising in the guinea pig under the study conditions.
Key value for chemical safety assessment
Skin sensitisation
Link to relevant study records
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: as per mentioned below
- Principles of method if other than guideline:
- The skin-sensitising potential of m-chloronitrobenzene was investigated in Dunkin-Hartley albino guinea pigs in the Magnusson and Kligman maximisation test
- GLP compliance:
- not specified
- Type of study:
- guinea pig maximisation test
- Justification for non-LLNA method:
- not specified
- Species:
- guinea pig
- Strain:
- Dunkin-Hartley
- Sex:
- not specified
- Details on test animals and environmental conditions:
- Age at study initiation: 7 to 8 weeks old
- Route:
- other: Intradermal injection
- Vehicle:
- other: Ethanol
- Concentration / amount:
- 0.1-percent m-chloronitrobenzene with 0.1 ml Freunds’ adjuvant, diluted with ethanol.
- Route:
- other: Epidremal
- Vehicle:
- other: Ethanol
- Concentration / amount:
- 0.1-percent m-chloronitrobenzene with 0.1 ml Freunds’ adjuvant, diluted with ethanol.
- No. of animals per dose:
- Group of 20 animals
- Details on study design:
MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures:
- Exposure period: One week
- Test groups: group of 20 animals
- Control group: Yes
- Site:No data available
- Frequency of applications: Once
- Duration: 1 week
- Concentrations: 0.1% with 0.1 ml Freunds’ adjuvant, diluted with ethanol.
B. CHALLENGE EXPOSURE
- No. of exposures:
- Day(s) of challenge:
- Exposure period: 24 hrs
- Test groups:
- Control group:
- Site:
- Concentrations: 25-percent ethanol solution of the chemical
- Evaluation (hr after challenge):
Other:A second challenge was carried out in the same way after another 2 weeks.- Challenge controls:
- Yes treated with ethanol alone.
- Positive control substance(s):
- not specified
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 0.1 and 25 %
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Clinical observations:
- No data available
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 24.0. Group: test group. Dose level: 0.1 and 25 %. No with. + reactions: 0.0. Total no. in groups: 20.0. Clinical observations: No data available.
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- No reactions were observed in any of the cases so that m-chloro-nitrobenzene therefore it was evaluated as nonsensitising in the guinea pig under the study conditions.
- Executive summary:
The skin-sensitising potential of m-chloronitrobenzene was investigated in Dunkin-Hartley albino guinea pigs (DUHA KFM strain, 7 to 8 weeks old) in the Magnusson and Kligman maximisation test. Groups of 20 animals were given intradermal injections of 0.1 ml Freunds’ adjuvant, diluted with etha-nol, and 0.1-percent m-chloronitrobenzene or just the adjuvant without test substance (control). One week thereafter, the animals which had been ex-posed to m-chloronitrobenzene were treated epidermally with a 25-percent ethanol solution of the chemical by affixing a soaked filter paper to the skin for 48 hours by means of a semi-occlusive dressing. The controls received ethanol alone. In order to assess the skin sensitising potential, a challenge treatment with 25-percent m-chloronitrobenzene or ethanol was carried out again after 2 weeks, but in this case the dressing was only left on for 24 hours. A second challenge was carried out in the same way after another 2 weeks. No reactions were observed in any of the cases so that m-chloro-nitrobenzene was evaluated as nonsensitising in the guinea pig.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not sensitising)
- Additional information:
The skin-sensitising potential of m-chloronitrobenzene was investigated in Dunkin-Hartley albino guinea pigs (DUHA KFM strain, 7 to 8 weeks old) in the Magnusson and Kligman maximisation test. Groups of 20 animals were given intradermal injections of 0.1 ml Freunds’ adjuvant, diluted with etha-nol, and 0.1-percent m-chloronitrobenzene or just the adjuvant without test substance (control). One week thereafter, the animals which had been ex-posed to m-chloronitrobenzene were treated epidermally with a 25-percent ethanol solution of the chemical by affixing a soaked filter paper to the skin for 48 hours by means of a semi-occlusive dressing. The controls received ethanol alone. In order to assess the skin sensitising potential, a challenge treatment with 25-percent m-chloronitrobenzene or ethanol was carried out again after 2 weeks, but in this case the dressing was only left on for 24 hours. A second challenge was carried out in the same way after another 2 weeks. No reactions were observed in any of the cases so that m-chloro-nitrobenzene was evaluated as nonsensitising in the guinea pig.
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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