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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 204-496-1 | CAS number: 121-73-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- fish early-life stage toxicity
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Long term study was performed to test the effects of 1 Chloro-3-nitrobenzeneon early life stages of Zebra fish.Based on the teratogenic effects the EC50 was determined.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
- log Pow: 2.59 - Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations:10- 100mg/l
- Vehicle:
- yes
- Details on test solutions:
- - Chemical name of vehicle (organic solvent, emulsifier or dispersant):
Stock solutions of the compounds were prepared in dimethylsulfoxide DMSO - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish/Zebra Danio
- Age at study initiation (mean and range, SD):<4h
- Feeding during test: No feeding during test - Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 7 d
- Hardness:
- 250mg/l
- Test temperature:
- 25 deg celcius
- pH:
- 8.2
- Dissolved oxygen:
- No data available
- Salinity:
- No data available
- Nominal and measured concentrations:
- No data available
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100ml glass dishes
- No. of organisms per vessel: 25 eggs per jar
- Renewal rate of test solution (frequency/flow rate): Alternate day
(Test solutions were refreshed every Monday, Wednesday and Friday).
-Photoperiod:12hr - Reference substance (positive control):
- not specified
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 39 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Days to hatch and numbers hatched: Eggs hatch after 3-4 days
- Other biological observations Delayed or inhibited embryonic development was also remarkable. Teratogenic effects on larvae were observed in concentrations at lethal-levels. These effects were noticed as
deviations at the pectoral fins, abnormalities of the vertebrae and
size-increase of the yolk sac. - Results with reference substance (positive control):
- No data available
- Reported statistics and error estimates:
- X2 test
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Delayed or inhibited embryonic development was also remarkable. Teratogenic effects on larvae were observed in concentrations at lethal-levels. These effects were noticed as
deviations at the pectoral fins, abnormalities of the vertebrae and
size-increase of the yolk sac. Based on the effects the EC50 was determined to be 39mg/l. Classification may not be done as it is a chronic study. - Executive summary:
Freshly spawned eggs were obtained from a lab-culture. Within 4h after fertilization eggs were introduce to dishes containing test solution. Exposure duration was 7 days. Eggs were observed daily for abnormal changes.
Specific temperature ph and hardness were maintained and fishes were not feeded during the study period. The dose range used in 10-100mg/l, At given Effect concentration (39mg/l) some teratogenic and physiological abnormalities were observed predicting the toxicity of substance to fish early life stages.
Reference
Highest mortality was observed in egg-stage
Description of key information
Delayed or inhibited embryonic development was also remarkable. Teratogenic effects on larvae were observed in concentrations at lethal-levels. These effects were noticed as
deviations at the pectoral fins, abnormalities of the vertebrae and
size-increase of the yolk sac. Based on the effects the EC50 was determined to be 39mg/l. Classification may not be done as it is a chronic study.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Long term toxicity to fish:
Based on the value aquired from peer reviewed journal,long term toxicity of fish was discribed as below:
Freshly spawned eggs were obtained
from a lab-culture. Within 4h after fertilization eggs were introduce to
dishes containing test solution. Exposure duration was 7 days. Eggs were
observed daily for abnormal changes.
Specific temperature ph and hardness were maintained and fishes were not
feeded during the study period. The dose range used in 10-100mg/l, At
given Effect concentration (39mg/l) some teratogenic and physiological
abnormalities were observed predicting the toxicity of substance to fish
early life stages.
Chronic study has been conducted for 7 days to test the effects of 1-chloro-3-nitrobenzene on danio rario.
Based on mortality observed for 7 days, the LC50 was determined to be 56 mg/l. Other than mortality some Delayed or inhibited embryonic development was also remarkable. Teratogenic effects on larvae were observed in concentrations at lethal-levels. These effects were noticed as deviations at the pectoral fins, abnormalities of the vertebrae and size-increase of the yolk sac.
Chronic study has been conducted for 14 days to test the effects of 1-chloro-3-nitrobenzene on danio rario.Based on mortality observed for 14 days, the LC50 was determined to be 15.4mg/l.
Chronic study has been conducted for 14 days to test the effects of 1-chloro-3-nitrobenzene onp reticullata.Based on mortality observed for 14 days, the LC50 was determined to be 20 mg/l & EC50 was determined to be 2.0 mg/l.
Based on the effect concentrations we can consider that the test substance 1-chloro-3-nitrobenzene has no chronic effect on fish and can be considered as not classified as per CLP.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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