Reaction products of amines, dicoco alkyl and glycollic acid

Endpoint summary

Additional information

Acute studies were available on two different trophic levels (fish, and invertebrates), and chronic studies were available also on two trophic levels (invertebrates, and algae). A prolonged toxicity study (14 -d) is available on fish. Moreover, a study on microorganims has been performed.

Acute studies

Fish:

The 96–hr-acute toxicity of test substance to Oncorhynchus mykiss was studied under static conditions. Fish were exposed to control, and test chemical at the nominal 100, 180, 320, 560, and 1000 mg/L loading rates for 96 hr. Mortality and sublethal effects were observed daily. The 96– hour LL₅₀ was 610 mg/L. The 96– hr NOELR based on mortality was 180 mg/L.

Daphnia:

The 48–hr-acute toxicity of test substance to Daphnia magna was studied under static conditions. Daphnids were exposed to control, and test chemical at the nominal 13, 22, 36, 60, 100 mg/L loading rates for 48 hr. Immobilization effects were observed daily. The 48– hour EC₅₀ was 77 mg/L. The 48– hr NOEC based on immobilization was 13 mg/L.

The 48–hr-acute toxicity of test substance to Daphnia magna was studied under static conditions. Daphnids were exposed to control, and test chemical at the nominal 100, 180, 320, 560, and 1000 mg/L loading rates for 48 hr. Immobilization effects were observed daily.

Due to the complex nature of WAFs and the observed changes in peak profile between the WAFs and the analytical standards, the results calculated from the GC and the TOC analyses should be viewed with caution as these may not be reliable indicators of the concentration of the test material present in the WAFs. The use of nominal loading rates is therefore considered a more appropriate indicator for the expression of the results of this study.

Nominal Loading rates: The 48– hour EC50 was 180mg/L loading rate WAF. The 48– hr NOEC based on immobilization was 100 mg/L loading rate WAF.

Algae

In a 72 hour acute toxicity study, the cultures of Scenedesmus subspicatus were exposed to test material at nominal concentrations of 0, 10, 20, 40, 80, and 160 mg/L under static conditions in accordance with the OECD guideline 203.

The EL50 and NOELR values based on cell density were > 160 and 20 mg/L nominal loading rate concentrations, respectively.

Considering these results, Daphnia is considered as the most sensitive species in the acute studies.

Prolonged toxicity

In a 14 -day toxicity study performed to Chinese study Guidelines Zebra fish (10 per test group) were exposed to nominal test concentrations of 54 or 108 mg/L Loading Rate WAF under semi-static conditions.

There were no mortalities in either the test groups or the blank control. No abnormalities in behaviour or morphology were observed in any group. The results of a positive control and a blank control confirm the validity of the test system.

The LC50 (14 d) in Zebra fish is > 108 mg/L Loading Rate WAF

 

Chronic studies :

The 21–d chronic toxicity of test substance to Daphnia magna was studied under semi-static conditions. Daphnids were exposed to control, and test chemical at the nominal 1.8, 5.6, 18, 56, and 180 mg/L loading rates for 21 d. Immobilization and reproductive effects were observed daily.

Given that toxicity cannot be attributed to a single to a single component or mixture of components but to the test material as a whole, the results were based on nominal loading rates only.

The 21 -d EL50 (immobilisation) was 100 mg/L. The 21 -d EL50 (reproduction) was 89 mg/L.The 21 -d NOELR based on immobilization and reproduction was 56 mg/L.

Algae is considered as the most sensitive species in the two available chronic studies.

 

Microorganisms study:

A study was performed to assess the effect of the test material on the respiration of activated sewage sludge. The method followed that described in the OECD 209. Following a preliminary range-finding test, activated sewage sludge was exposed to an aqueous dispersion of the test material at a concentration of 1000 mg/L for a period of 3 -h at 21°C with the addition of a synthetic sewage as a respiratory substrate.

 

The effect of the test material on the respiration of activated sewage sludge gave a 3h-EC50 > 1000 mg/L. The 3h-NOEC was 1000 mg/L.