Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 227-815-6 | CAS number: 5989-54-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- the study does not need to be conducted because the substance is found to be readily biodegradable and the applied test concentrations are in the range of concentrations that can be expected in the influent of a sewage treatment plant
- Justification for type of information:
- JUSTIFICATION FOR DATA WAIVING
According to column 2 of REACH Annex VIII, the study does not need to be conducted on microorganisms because the substance is found to be readily biodegradable and the applied test concentrations (2 mg/L) are in the range of concentrations that can be expected in the influent of a sewage treatment plant ( 1.60E-19< PEC stp< 2.66E-02 mg/L). - Reason / purpose for cross-reference:
- data waiving: supporting information
Reference
Description of key information
Weight of evidence: One valid ready biodegradability study on l-limonene and one valid activated sludge respiration test on dipentene multi-constituents (48.4% dl-limonene) were available to assess the toxicity of l-limonene to microorganisms. The most sensitive value, a NOEC of 2 mg/L derived from the ready biodegradability test, was considered as the reliable value for risk assessment purposes.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 2 mg/L
Additional information
According to column 2 of REACH Annex VIII, the study does not need to be conducted on microorganisms because the substance is found to be readily biodegradable and the applied test concentration (2 mg/L) was higher that can be expected in the influent of a sewage treatment plant ( 1.60E-19< PEC stp< 2.66E-02 mg/L).
According to ECHA, Chapter R.7b, Endpoint specific guidance (2008), the information content of a ready biodegradability test can be used to derive a NOEC. In the case of l-limonene, the close bottle test using unadapted domestic sludge (5989-54-8, Biodegradation in water: screening tests, van Ginkel, 2010, RS, K) according to OECD 310 (2006) and ISO 10707 (1994) fulfills the following requested conditions: the compound is found to be readily biodegradable and the test substance concentration (2 mg/L) was not above the solubility limit of l-limonene (12.3 mg/L). A toxicity control that shows good degradation of the positive control substance (sodium acetate) in the presence of the test substance haven't been included in this test because it was believe that possible toxicity of l-limonene to microorganisms degrading acetate was not relevant. However inhibition of the endogenous respiration of the inoculum by the test substance was not detected and therefore, no inhibition of the biodegradation due to the "high" initial concentration of the test substance is expected. This hypothesis is reinforced by the result of a 3 hours acute toxicity study performed in accordance with the OECD guideline 209, on unadapted domesticactivated sludge exposed to Dipentene multi-constituent (dl-limonene purity was 48,4%) at nominal concentrations of 0 (control), 8.45, 16.9, 33.8, 67.6, 135.2, 270.4, 540.8 mg/L under static conditions.
The EC50 and EC10values for the respiration rate inhibition were 209 and 18 mg test item/L, respectively and the test concluded that the test item was not harmful to activated sludge. In conclusion a NOEC of 2 mg/L corresponding to a good degradated tested concentration can be derived from the ready biodegradability test for risk assessment purposes.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)