2,6-di-tert-butyl-4-(4,6-bis(octylthio)-1,3,5-triazin-2-ylamino)phenol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18-03-1985 to 22-03-1985

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non GLP study. Deviations from the updated OECD-guideline (adopted 1992): Highest vehicle concentration: > 100 mg/L

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

: highest vehicle concentration: 954 mg/L

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

- Sampling method: Samples for analysis were taken at 0 h and 96 h exposure. Approx. 50 ml of testwater were taken after intensively stirring and filled in a tared bottle containing 30 mL of tetrahydrofuran. The bottle was reweighed. The resulting sample weight was converted to volume.

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Stock solution: 100 g test substance were dissolved in DMF; 4000 mg MARLOPON AT50 and were made up to 1000 nl with DMF
- Controls: Water and Vehicle
- Chemical name of vehicle: DMF and Marlopon AT50
- Concentration of vehicle in test medium: Controls: 950 mg DMF and 4 mg Marlopon AT50 per liter water in the concentration used for the highest test concentration
- Evidence of undissolved material: A deposit was observed within 2h after mixing.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Source: West Aquarium / 37431 Bad Lauterberg
- Length at study initiation: 25 mm (20-28 mm)
- Weight at study initiation: 0.13 g (0.09-0.18 g)
- Feeding during test: none

ACCLIMATION
- Acclimation period: 34 days

ADAPTATION
-no food 24 hours prior to exposure

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

180 mg CaCO3/L

Test temperature:

23 +- 1 °C

pH:

8.0

Dissolved oxygen:

Blank: 92 - 100 %
Vehicle: 65 - 100 %
Test concentrations: 70 - 100 %

Nominal and measured concentrations:

100 mg/L (nominal); 72 mg/L (measured)

Details on test conditions:

TEST SYSTEM
- Test vessel: Aquaria
- Material, size, headspace, fill volume: glass; 20 L (36/22/25 cm); 15 L
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.10 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water (carbon filter)
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Photoperiod: Fluorescent light, 16 h daily

EFFECT PARAMETERS MEASURED: mortality and visible abnormalities after 24, 48, 72, 96 h

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: none
- Other biological observations: none
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: yes, a deposit was observed 2h after mixing
- Effect concentrations exceeding solubility of substance in test medium: yes

Description of key information

With high probability acutely not harmful to fish. 

Additional information

The acute toxicity to fish was assessed in a 96-h acute toxicity study to zebra-fish (Brachydanio rerio) in accordance to OECD guideline 203. The test was conducted under static conditions with a nominal concentration of 100 mg/L. The 96-h LC50 was determined to be > 100 mg/L.