2,6-di-tert-butyl-4-(4,6-bis(octylthio)-1,3,5-triazin-2-ylamino)phenol

Basic toxicokinetics

Administrative data

Endpoint:

basic toxicokinetics in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1966

Report date:

1966

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1965

Report date:

1965

Materials and methods

Objective of study:

absorption

Principles of method if other than guideline:

The absorption of the compound was evaluated by the Levine technique, using C-labeled materials as described in the Journal of Pharmacology and Experimental Therapeutics, 114:78 (1955), 121019 (1961).

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,6-di-tert-butyl-4-(4,6-bis(octylthio)-1,3,5-triazin-2-ylamino)phenol

EC Number:

213-590-1

EC Name:

2,6-di-tert-butyl-4-(4,6-bis(octylthio)-1,3,5-triazin-2-ylamino)phenol

Cas Number:

991-84-4

Molecular formula:

C33H56N4OS2

IUPAC Name:

2,6-di-tert-butyl-4-(4,6-bis(octylthio)-1,3,5-triazin-2-ylamino)phenol

Specific details on test material used for the study:

- Name of test material (as cited in study report): RA 565

Radiolabelling:

yes

Remarks:

C14

Test animals

Species:

rat

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Fasting period before study: Yes

Administration / exposure

Route of administration:

other: injected into gut lumen

Vehicle:

CMC (carboxymethyl cellulose)

Details on exposure:

PREPARATION OF DOSING SOLUTIONS: Suspension in 1% carboxymethylcellulose solution was made by sonication.

Duration and frequency of treatment / exposure:

single introduction

Doses / concentrations

Remarks:

Doses / Concentrations:
891, 865, and 907 µg

No. of animals per sex per dose / concentration:

1

Control animals:

no

Details on dosing and sampling:

PHARMACOKINETIC STUDY (Absorption)
A small abdominal opening was made in a lightly etherized, fasted rat and the upper end of the small intestine was exposed and ligated about 6 inches below the pylorus. A second ligature was placed loosely 5 inches below the first. The test material (as a solution 1 % carboxymethylcellulose) was introduced with a hypodermic syringe as follows: the needle was inserted into the gut lumen from a point beyond the second tie, the ligature was pulled tight around the barrel of the needle, the sample was injected and, as the needle was withdrawn, the ligature was secured. The abdominal incision was closed and the animal recovered.
The intestinal vasculature remained essentially intact, allowing absorbed material to be removed to the general circulation in the normal manner. After a suitable period of time, generally 3 hours, the rat was again etherized and the ligated intestinal segment was removed for determination of the amount of compound still present.

Results and discussion

Main ADME results

Main ADME results 1

Type:

absorption

Results:

The test substance is poorly absorbed.

Main ADME results 2

Type:

distribution

Results:

No data

Main ADME results 3

Type:

metabolism

Results:

No data

Main ADME results 4

Type:

excretion

Results:

No data

Any other information on results incl. tables

Tab. 1: Absorption of the test substance 3 hours after injection (1st series, 1965).

Dose (µg)	Amount recovered (µg)	Absorption (%)
891	879	1.3
865	835	3.5
907	812	10.5

Tab. 2: Absorption of the test material in the Levine Procedure (1st and 2nd series, 1965 and 1966)

1^stseries	10.5 %
	3.5 %
	1.3 %
2^ndseries	12.3 %
	2.5 %
	0.2 %
Average 1^stseries	5.1 %
Average 2^ndseries	5.0 %
Mean (all vaues)	5.0 ± 5.1 %
95% Confidence Limits	- 0.3 to 10.4 %