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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Endpoint summary

Administrative data

Description of key information

Additional information

One key study is available on the bioaccumulation of Amber Core on Carp (Cyprinus carpio). This study was performed in a flow through system under GLP and according to the "Method for Testing the Degree of Accumulation of Chemical Substances in Fish Body" stipulated in the "Testing Methods for New Chemical Substances" (July, 1974) and is based on OECD Guideline 305C (May, 1981).

An acute toxicity test was performed before the bioaccumulation test. This preliminary test result for 48 -hour LC50 (=9.95 mg/L) was used to decide on the test concentrations of the test substance in the bioaccumulation study. Two test medium concentration levels were used: 0.050 mg/L and 0.0050 mg/L. The temperature of test water was maintained at 25 +/- 2°C with concentrations of dissolved oxygen in test tank comprised between 6.4 and 7.8 mg/L (corresponding to >60% saturation). 15 fish were used in Level 1 and 2 and 5 fish were used in control (the control was set as a blank test). The duration of exposure was 8 weeks. 2 peaks on the mass fragmentogram were detected by GC-MS. The peaks were named peak A and B in the elution order respectively. The concentrations of test substance were analyzed by GC-MS. BCFs of the test substance were estimated to have reached equilibrium after 8 weeks. The bioaccumulation potential of test substance in carp was an approximately similar value at both levels.

However, to consider the worst case, the value used for chemical safety assessment was the highest BCF obtained: 173.

Furthermore, the partition coefficient of Amber Core was estimated using a QSAR calculation and the corresponding mean of 3.81 was retained as key value. Additionally, to provide supplementary evidence, the bioconcentration factor was also estimated using QSAR calculations. With EPI Suite – BCFBAF v3.01, the BCF was comprised between 218.8 L/Kg and 304.1 L/Kg. With the CAESAR model, the BCF was estimated to be 27 L/Kg. It is assumed that the affinity of the substance for the lipids of an organism is insufficient for it to be considered bioaccumulative.