Benzeneacetic acid, 4-(cyclopropylcarbonyl)-.alpha.,.alpha.-dimethyl-, ethyl ester

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

12 January to 9 April 1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study performed according to international guidelines.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

ISO DIS 9439 (Ultimate Aerobic Biodegradability - Method by Analysis of Released Carbon Dioxide)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-(Cyclopropylcarbonyl)-α,α-dimethyl-ethyl ester benzeneacetic acid

EC Number:

605-546-9

Cas Number:

169280-10-8

Molecular formula:

C16 H20 O3

IUPAC Name:

4-(Cyclopropylcarbonyl)-α,α-dimethyl-ethyl ester benzeneacetic acid

Details on test material:

Batch: Dest. 3-1, dated April 7, 1997, from PT/TTF
Code: HOE CG 0530 OY ZD99 0001
Purity: 99%
Date of delivery: August 27, 1997
C-content: 738.2 mg/g [C]

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Sediment from the activated sludge was washed two times by centrifuging and resuspending in mineral medium.
Amount: 10 ml sludge/L medium
Content of sludge sediment: 16 g/L
Content dry matter: 12 mg/L
Pretreatment: Aerated for 7 days

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

122.8 other: mg/vessel

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

122.5 other: mg/vessel

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

Carbon content in the test system: 90.65 mg/batch [C] and 90.43 mg/batch [C]

Reference substance

Reference substance:

benzoic acid, sodium salt

Remarks:

pH=7.2, DOC= 583.4 mg/g, amount: 50ml/l, concentration used: 29.17 mg/l

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

3

Sampling time:

28 d

Remarks on result:

other: VESSEL A= 2.6%; VESSEL B=3.7%

Details on results:

POSITIVE CONTROL: 63% degradation within 9 days and 77% within 28 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

p-Cyclopropylketoester is not readily biodegradable under the conditions of this test. Significant biodegradation was not detected.