1-isopropyl-4-methylcyclohexane

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23/04/1992 to 07/05 1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: limited information on the soil used, not indicated whether outcome is based on soil wet weight or dry weight.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)

Version / remarks:

302/88 EWG

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

p-menthane

IUPAC Name:

p-menthane

Constituent 2

Reference substance name:

1-isopropyl-4-methylcyclohexane

EC Number:

202-790-4

EC Name:

1-isopropyl-4-methylcyclohexane

Cas Number:

99-82-1

Molecular formula:

C10H20

IUPAC Name:

1-methyl-4-(propan-2-yl)cyclohexane

Details on test material:

- Name of test material (as cited in study report): p-menthane
- Physical state: liquid

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Test organisms

Test organisms (species):

Eisenia fetida

Details on test organisms:

TEST ORGANISM
- Common name: Eisenia foetida foetida
- Source: Eisenia GmbH und Co, 6200 Wiesbaden
- Age at test initiation (mean and range, SD): >2 months
- Weight at test initiation (mean and range, SD): 300 - 605 mg

ACCLIMATION
- Acclimation conditions: kept on 50% peat and 50% horse or bovine manure

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Test conditions

Test temperature:

mean 21 °C

pH:

6.1 - 6.5 (6.7 at 1000 mg/kg, all worms died within 7 days)

Moisture:

40 - 41%

Details on test conditions:

TEST SYSTEM
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil): no data.

OTHER TEST CONDITIONS: no data

EFFECT PARAMETERS MEASURED : mortality

Nominal and measured concentrations:

nominal: 100, 180, 300, 550, 1000 mg/kg soil

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

7 d

Dose descriptor:

LC100

Effect conc.:

1 000 other: mg/kg soil

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

7 d

Dose descriptor:

NOEC

Effect conc.:

300 other: mg/kg soil

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 3

Duration:

14 d

Dose descriptor:

LC100

Effect conc.:

1 000 other: mg/kg soil

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 4

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

300 other: mg/kg soil

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Results with reference substance (positive control):

LC100 (7 - 14 d) 1000 mg/kg soil

Reported statistics and error estimates:

probit

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test substance did not induce mortality in Eisenia foetida at concentrations of 300 mg/kg soil and below.

Executive summary:

Earthworms (Eisenia foetida) were exposed to the test substance in soil at nominal concentrations of 100, 180, 300, 550, 1000 mg/kg soil. The NOEC based on mortality was 300 mg/kg soil.