1-isopropyl-4-methylcyclohexane

Toxicity to terrestrial plants

Administrative data

Endpoint:

toxicity to terrestrial plants: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23/3 1992 to 10/4 1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: similar to guideline study under GLP, but no analytical confirmation of test concentrations and limited information on soil used

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-isopropyl-4-methylcyclohexane

EC Number:

202-790-4

EC Name:

1-isopropyl-4-methylcyclohexane

Cas Number:

99-82-1

Molecular formula:

C10H20

IUPAC Name:

1-methyl-4-(propan-2-yl)cyclohexane

Constituent 2

Reference substance name:

p-Menthane

IUPAC Name:

p-Menthane

Details on test material:

- Name of test material (as cited in study report): p-menthane
- Physical state: liquid
- Stability under test conditions: stable (no details)

No additional information available

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Test organisms

1

Species:

Triticum aestivum

Plant group:

Monocotyledonae (monocots)

2

Species:

Trifolium ornithopodioides

Plant group:

Dicotyledonae (dicots)

3

Species:

Lepidum sativum

Plant group:

Dicotyledonae (dicots)

Study design

Test type:

other: seedling emergence and growth test

Study type:

laboratory study

Substrate type:

natural soil

Limit test:

no

Total exposure duration:

18 d

Post exposure observation period:

none

Test conditions

Test temperature:

day 25 °C; night 20 °C

pH:

6.8

Moisture:

15% water added, measured 21 - 22%

Details on test conditions:

TEST SYSTEM
- Test container (type, material, size): plastic pot height 9 - 10 cm; Ø 8 cm
- Amount of soil: 200 g ww
- No. of seeds per container: 5
- No. of replicates per treatment group: 4
- No. of replicates per control: 5

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Soil texture (if natural soil): sand (USDA)
- % sand: 95
- % silt: 5 (partcles <20um)
- % clay: 0
- Organic carbon (%): 0.18%
- not sieved

GROWTH CONDITIONS
- Photoperiod: 10/14 (L/D)
- Light source: Hg lamp
- Light intensity: growth phase day 10000 Lux; emergence phase ca. 300 Lux
- Day/night temperatures: 25/20 °C
- Relative humidity (%): 100%
- Air exchange rate: not indicated
- Watering regime and schedules: not indicated
- Volume applied: 15%

EFFECT PARAMETERS MEASURED :
- Emergence, growth on day 18 by fresh shoot weight
- Phytotoxicity rating system: none

VEHICLE CONTROL PERFORMED: yes

No additional information provided

Nominal and measured concentrations:

nominal: 1, 10 and 100 mg/kg dw

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Species:

Triticum aestivum

Dose descriptor:

NOEC

Effect conc.:

100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks on result:

other: in controls 17/20

Effect concentrations 2

Species:

Triticum aestivum

Duration:

18 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Effect concentrations 3

Species:

Trifolium ornithopodioides

Dose descriptor:

NOEC

Effect conc.:

100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks on result:

other: in controls 20/20 seeds emerged

Effect concentrations 4

Species:

Trifolium ornithopodioides

Duration:

18 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Effect concentrations 5

Species:

Lepidum sativum

Dose descriptor:

NOEC

Effect conc.:

100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks on result:

other: in controls 17/20

Effect concentrations 6

Species:

Lepidum sativum

Duration:

18 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Any other information on results incl. tables

see attached document

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test substance did not induce toxicity to emerging and growing seedlings of three plant species.

Executive summary:

In order to assess effects of the test substance on emergence and growth of seedlings of Triticum aestivum, Trifolium ornithopodioides and Lepidum sativum, seeds were planted and allowed to grow for 18 days. No effects on emergence and growth were seen at any of the concentrations tested (1, 10, 100 mg/kg dw mixed in soil).