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Diss Factsheets
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EC number: 289-064-0 | CAS number: 85959-68-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- adult fish: sub(lethal) effects
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment; however there were no analytical measurements of test solution concentrations.
- Justification for type of information:
- 1. HYPOTHESIS FOR THE ANALOGUE APPROACH
The effect observed for DTPA-H5 during a Long term fish test as published by van Dam is read across to DTPA-Fe(NH4)2. This read across is considered justified because the effects observed will be caused by the DTPA-Fe complex. Ammonium, potassium, sodium, hydrogen are only counter ions needed to neutralize the product and these ions will easily dissociate from the DTPA-Fe complex when evaluated in aquatic ecotoxicity tests under ambient conditions. REPORTING FORMAT FOR THE ANALOGUE APPROACH
[Please provide information for all of the points below. Indicate if further information is included as attachment to the same record, or elsewhere in the dataset (insert links in - Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Adult fish (2 males and three females per tank) were exposed to the test material for 28 days, and reproduction, growth, organ weights, and an enzymatic biomarker were assessed.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
no data - Analytical monitoring:
- no
- Details on sampling:
- not applicable
- Vehicle:
- no
- Details on test solutions:
- The experiment was carried out using a static-renewal regime, whereby 50% of the test solution was replaced daily. Test solutions for each aquarium were prepared daily in a 20-L plastic container using a freshly prepared 5-liter stock solution of 2000 mg/L DTPA. The dilution water used in the experiment was wet laboratory water which was pumped into a 400-liter storage tank and heated to 25 degrees C. The pH of the stock solution and the test solutions was adjusted to approximately 7.4 with 5 M NaOH.
- Test organisms (species):
- other: Melanotaenia fluviatilis
- Details on test organisms:
- TEST ORGANISM
- Common name: Crimson-spotted Rainbowfish
- Source: The original M. fluviatilis laboratory stocks were collected from the Murray River, Victoria, Australia in 1990.
- Method of breeding:
- Feeding during test
- Food type: flake food, live and frozen brine shrimp
- Amount: ad libitum (flake food), 5 -ml (brine shrimp)
- Frequency: 2 times daily
ACCLIMATION
- Acclimation period: 1 week
- Acclimation conditions (same as test or not): same as in the test - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 28 d
- Post exposure observation period:
- Embryos were collected from the exposure aquaria, put into clean water and observed for viability.
- Hardness:
- not reported
- Test temperature:
- 25 degrees C
- pH:
- mean pH after renewals = 7.31 (standard error = 0.03)
mean pH prior to renewals = 7.28 (standard error = 0.03) - Dissolved oxygen:
- Mean DO in test aquaria = 8.20 (standard error = 0.07)
Mean DO in egg-hatching aquaria = 9.11 (standard error = 0.06) - Salinity:
- not applicable
- Nominal and measured concentrations:
- Nominal concentrations were 0, 1, 10, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria containing 30 liters of aerated test solution.
- Renewal rate of test solution (frequency/flow rate): 50% renewal (15 liters) daily, except on Day 15, when there was a 100% test solution renewal
- No. of organisms per vessel: 5 (2 males and 3 females)
- No. of vessels per concentration (replicates): 5 replicates
- No. of vessels per control (replicates): 5 replicates
OTHER TEST CONDITIONS
- Adjustment of pH: pH adjusted to 7.4
- Conductivity ranged from 54.6 (control) to 104.7 (100 mg/L) uS/cm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Reproduction was assessed on Day 0, 6-7, 13-14, 27-28.
At the end of the exposure (Day 28) fish length and weight were measured. Gonad and liver weight were also determined on Day 28 to calculate the liver-somatic index and the gonadal-somatic index. Ethoxyresorufin O-deethylase (EROD) activity was also assessed in fish liver tissue following 28 days of exposure.
- Reference substance (positive control):
- no
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- The only effect observed during the experiment was a 50% inhibition in activity of the hepatic MFO enzyme ethoxyresorufin O-deethylase in female rainbow fish exposed to 100 mg/L DTPA. This effect is biochemical in nature and is not an appropriate endpoint on which to base risk assessment decisions. No effects on condition factor or reproduction were noted at the highest concentration of 100 mg/L DTPA.
- Results with reference substance (positive control):
- no data
- Reported statistics and error estimates:
- no data
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC (based on reproduction) was 100 mg/L.
- Executive summary:
Adult male and female crimson-spotted rainbowfish were exposed to DTPA in a static-renewal system for a period of 28 days and their reproduction, LSI, GSI, condition factor and EROD activity were assessed. There were no effects of parental DTPA exposure on apical endpoints, such as reproduction or condition factor. The only effect observed during the experiment was a 50% inhibition in activity of the hepatic MFO enzyme ethoxyresorufin O-deethylase in female rainbowfish exposed to 100 mg/L DTPA. This effect is biochemical in nature and is not an appropriate endpoint on which to base risk assessment decisions. Thus, the overall NOEC for the apical endpoints measured in this study (growth, reproduction) is 100 mg/L DTPA.
Reference
Description of key information
The 28-day NOEC for reproduction in the crimson-spotted rainbowfish (Melanotaenia fluviatilis) was 100 mg/L DTPA (free acid), which is equivalent to 123 mg/L DTPA-Fe(NH4)2.
The effect observed for DTPA-H5 during a long term fish test as published by van Dam (1999) is read across to DTPA-Fe(NH4)2. This read across is considered justified because the effects observed will be caused by the DTPA-Fe complex. Ammonium, potassium, sodium, hydrogen are only counter ions needed to neutralize the product and these ions will easily dissociate from the DTPA-Fe complex when evaluated in aquatic ecotoxicity tests under ambient conditions.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 123 mg/L
Additional information
One chronic study for fish was available for DTPA (free acid). This study was well documented and met generally accepted scientific principles, and thus, was considered acceptable for assessment. In this study, adult male and female crimson-spotted rainbowfish (Melanotaenia fluviatilis) were exposed to DTPA (free acid) in a static-renewal system for a period of 28 days. Upon contact with water, pentasodium DTPA is expected to dissociate, thus, is it considered essentially equivalent to the free acid form of DTPA in aquatic environments. The endpoints that were measured in this study included reproduction, liver-somatic index, gonadal-somatic index, condition factor and EROD activity. There were no effects of parental DTPA (free acid) exposure on apical endpoints, such as reproduction or condition factor, at the highest test concentration of 100 mg/L DTPA (free acid), which, following stoichiometric conversion, is equivalent to 123 mg/L DTPA-Fe(NH4)2.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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