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Diss Factsheets
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EC number: 206-022-9 | CAS number: 288-88-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
- Report date:
- 1976
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- reliability scoring based on 2002 guideline
- Deviations:
- yes
- Remarks:
- Source, purity, chemical characteristics of test substance, temperature, light cycle, and humidity not provided; grading scale for irritation not reported
- GLP compliance:
- no
- Remarks:
- study pre-dates GLP requirements
Test material
- Reference substance name:
- 1,2,4-triazole
- EC Number:
- 206-022-9
- EC Name:
- 1,2,4-triazole
- Cas Number:
- 288-88-0
- Molecular formula:
- C2H3N3
- IUPAC Name:
- 1H-1,2,4-triazole
- Details on test material:
- - Name of test material (as cited in study report): 1,2,4-triazole
- Analytical purity: reported as technically pure
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: from the breeder Winkelmann (Borchen)
- Age at study initiation: no data
- Weight at study initiation: 3-4kg
- Fasting period before study: no data
- Housing: singly in standard rabbit cages
- Diet (e.g. ad libitum): Rabbit food 222 (Hoeing co.), ad libitum
- Water (e.g. ad libitum): not precised, ad libitum
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS: no data
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- Test article was instilled into the conjuctival sac of the left eye of 2 rabbits at a dose of 50 mg/animal.
- Duration of treatment / exposure:
- Single administration
- Observation period (in vivo):
- 7 days
- Number of animals or in vitro replicates:
- 2
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Remarks:
- 2 rabbits
- Time point:
- 24/48 h
- Score:
- 0.5
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks on result:
- other: (1 rabbit)
- Irritation parameter:
- iris score
- Basis:
- mean
- Remarks:
- 2 rabbits
- Time point:
- 24/48 h
- Score:
- 0.5
- Max. score:
- 2
- Reversibility:
- fully reversible
- Remarks on result:
- other: (1 rabbit)
- Irritation parameter:
- conjunctivae score
- Remarks:
- (redness)
- Basis:
- mean
- Remarks:
- 2 rabbits
- Time point:
- 24/48 h
- Score:
- 2.5
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Remarks:
- 2 rabbits
- Time point:
- 24/48 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks on result:
- other: (1 rabbit)
- Irritant / corrosive response data:
- One hour after application intense reddening and a very intense swelling of the conjectivae of the treated eyes had developed. 24 hours after application intense reddening and moderate swelling of the conjuctivae was still present. In one animal, there was still slight redness and swelling of the conjuctivae 5 days after application, while it was normal in the other animal. The conjuctivae of both animals were normal after 7 days. During the first and second days after application, a slight, dispersed, diffuse opacity of the cornea was observed. The iris was slightly reddened and swollen.
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 (irritating to eyes) based on GHS criteria
- Conclusions:
- The authors concluded that 1,2,4-triazole has a irritant effect on the mucous membrane.
- Executive summary:
A non GLP comparable Guideline 405 (Acute Eyes Irritation / Corrosion) test was carried out with 1,2,4 -triazole.
Undiluted test article was instilled into the conjuctival sac of the left eye of 2 rabbits at a dose of 50 mg/animal.
One hour after application intense reddening and a very intense swelling of the conjectivae of the treated eyes had developed. 24 hours after application intense reddening and moderate swelling of the conjuctivae was still present. In one animal, there was still slight redness and swelling of the conjuctivae 5 days after application, while it was normal in the other animal. The conjuctivae of both animals were normal after 7 days. During the first and second days after application, a slight, dispersed, diffuse opacity of the cornea was observed. The iris was slightly reddened and swollen.
Under the experimental conditions, the test substance is irritant and is classified in category 2 according to regulation EC no.1272/2008 (CLP).
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