Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-800-2 | CAS number: 126-73-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 2020
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Tri-n-butyl phosphate induced earthworm intestinal damage by influencing nutrient absorption and energy homeostasis of intestinal epithelial cells
- Author:
- Yang Yang, Peng Liu, Mei Lia
- Year:
- 2 020
- Bibliographic source:
- Journal of Hazardous Materials 398 (2020) 122850
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Version / remarks:
- Version: 1984
- GLP compliance:
- no
Test material
- Reference substance name:
- Tributyl phosphate
- EC Number:
- 204-800-2
- EC Name:
- Tributyl phosphate
- Cas Number:
- 126-73-8
- Molecular formula:
- C12H27O4P
- IUPAC Name:
- tributyl phosphate
- Details on test material:
- No further information is reported.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- 500 mg/L TnBP was ultrasonic dispersed at 50 °C and sequentially dissolved with distilled water, 10 mL different concentrations of 500, 50 and 5 mg/L TnBP were added to 165 mL distilled water and then thoroughly mixed with the artificial soil to obtain desired TnBP concentration (0, 0.1, 1, and 10 mg/kg (dry
soil)). Different concentrations of TnBP (0, 0.1, 1, and 10 mg/kg (dry soil)) were prepared with distilled water and were thoroughly mixed with the artificial soil.
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- Earthworms cultured outdoor were obtained from a local farming factory (Jurong, China) and acclimatized at 20 ± 1 °C for at least one week prior to avoid interference caused by external environment and help earthworms adapt to the exposed environment. After acclimatization, healthy adult earthworms with clear clitellum (weighing approximately 500−600 mg) were chosen for artificial soil test.
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Total exposure duration:
- 14 d
Test conditions
- Test temperature:
- 20 ± 1 °C
- Moisture:
- final soil moisture 35 % (w/w)
- Details on test conditions:
- Artificial soil tests were conducted in 1 L clear glass bottles with 500 g dry artificial soil (final soil moisture 35 % (w/w)) following the OECD guidelines (OECD, 1984).
After the prepared soil had stabilized at 20 ± 1 °C for one week, 10 earthworms (acclimatized to artificial soil for 24 h) were placed in each container and kept in an incubator at 20 ± 1 °C in the dark for 14 days. Each concentration consisted of six bottles/replicates. - Nominal and measured concentrations:
- nominal: 0, 0.1, 1, and 10 mg/kg (dry soil)
Results and discussion
- Details on results:
- A LC50 value was not reported.
Any other information on results incl. tables
The weight of earthworms increased in all treatments with time and multi way ANOVA identified a significant difference between biomass and time at the 0.05 level; however, no significant dose-related difference were observed between treatments.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The mortality in the controls did not exceed 10% at the end of either test.
- Conclusions:
- The artificial soil test showed no significant dose-related difference in mortality (lower than 5%) for the exposure of E. fetida with tributyl phosphate (nominal concentration: 0, 0.1, 1, and 10 mg/kg (dry soil)) over a period of 14 days.
- Executive summary:
Artificial soil tests similar to OECD TG 207 suggested that the acute toxicity of tributyl phosphate (TnBP) towards earthworm Eisenia fetida is quite low since no mortality occurred and a steady increase of biomass was observed.
The overall objective of the present study was to investigate the TnBP induced intestinal toxicity mechanism on earthworm Eisenia fetida as well as the potential role of gut bacteria on host’s health. TnBP was found to have high bioconcentrations in earthworm intestinal tract. Digestive tract degradation and digestive enzyme activities disruption associated with nutrients absorption were noticed. Using multi-omics approaches, detailed intestinal toxic mechanism of earthworms under TnBP exposure was provided.
Tight junctions between small intestinal epithelial cells and osmotic equilibrium were destroyed under 10 mg/kg TnBP, leading to nutrient absorption disturbance. To satisfy the excessive energy requirements induced by TnBP, amino acids gluconeogenesis and protein degradation were detected. Moreover, TnBP significantly decreased the diversity of gut microbiota and changed their structure and function involved in hosts’ health and nutrients supply.The long-term toxicity data was not generated according to the specifications set out in OECD TG222.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)