Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-316-0 | CAS number: 57-14-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06 September 2010 - 29 October 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Compliant to GLP and testing guideline; adequate coherence between data, comments and conclusions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N,N-dimethylhydrazine
- EC Number:
- 200-316-0
- EC Name:
- N,N-dimethylhydrazine
- Cas Number:
- 57-14-7
- Molecular formula:
- C2H8N2
- IUPAC Name:
- 1,1-dimethylhydrazine
- Details on test material:
- - Name of test material (as cited in study report): UDMH (Unsymmetrical Dimethylhydrazine)
- Physical state: liquid
- Analytical purity: 99.4%
- Impurities (identity and concentrations): water 0.2%, amines 0.4%, methanol <0.01%, chlorides 0.0022%, iron < 0.00006%, mechanical impurities < 0.0001%
- Purity test date: 10 september 2008
- Lot/batch No.: 1
- Expiration date of the lot/batch: 23 january 2012 (supplier's indication by e-mail)
- Storage conditions of test material: at room temperature.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all
- Sampling method: aliquot (approximately 10 mL)
- Sample storage conditions before analysis: analyzed <1h after sampling
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no, soluble
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: Pseudokirchnerella subcapitata
- Source (laboratory, culture collection): internal culture lab
ACCLIMATION: none
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- no
Test conditions
- Test temperature:
- 23°C +/- 2°C
- pH:
- 8.1 in test medium non-treated
- Nominal and measured concentrations:
- Theoretical: 0.21, 0.47, 1.03, 2.27 and 5 mg/L
Nominal (based on weighed amounts for preparations): 0.24, 0.54, 1.18, 2.60 and 5.74 mg/L
Measured (geometric average): 0.16, 0.32, 0.77, 1.78 and 4.08 mg/L - Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: 10000 cells per mL
- No. of vessels per concentration (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: demineralized water added with (Calcium, Ammonium, Manganese, Zinc, Cobalt and Magnesium) chlorides, Magnesium sulphate, Potassium dihydrogenocarbonate, iron, EDTA, Orthoboric acid, Sodium molybdate and Sodium hydrogenocarbonate.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod, intensity: continuous light at approximately 7000 lux
EFFECT PARAMETERS MEASURED: growth (derived from optical density) at 24, 48 and 72h - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.09 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 1.91-2.28 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.35 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 0.27-0.44 mg/L
- Results with reference substance (positive control):
- potassium dichromate August 30th, 2010: ErC50 = 1.36 mg/L [1.31 – 1.42]
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- For UDMH, ErC50 in algae was 2.09 mg/L over 72h.
- Executive summary:
UDMH was tested in static conditions for acute toxicity toward Pseudokirchneriella subcapitata. The ErC50 (growth rate) over 72h was 2.09 mg/L, based on measured time-weighed geometric average concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)