Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 208-754-4 | CAS number: 540-72-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 08 December 1998 - 30 December 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was performed according to internationally accepted guidelines and under GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Details on test material:
- Identification: Ammonium thiocyanate
Description: White crystalline solid
Chemical name: Thiocyanic acid, ammonium salt
Cas - no: 1762-95-4
Batch: 0609810070729
Purity: 99.9% pure (from CoA)
Test substance storage: In refrigerator in the dark
Stability under storage: stable
Expiry date: 14 October 2001
Density: 1300 kg /m3
Bulk density: 600 - 700 kg /m3
Specific Gravity: 1.3
Stability in vehicle: Water :at least 96 h
Rationale for vehicle: The vehicle was selected based on trial formulations performed at NOTOX and/or test substance data supplied by the sponsor.
Constituent 1
Test animals
- Species:
- other: Japanese quail (Coturnix coturnix faponica)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: H. und E. Kilberich, Geesdorf, Germany
- Age at study initiation: 8 weeks
- Weight at study initiation: males : 182 - 254 g of treatment females: 185 - 234 g. The weight of one male was slightly outside the range given in the protocol. This deviation was considered not to have affected the study integrity.
- Fasting period before study: Prior to dosing food was withheld for If to 20 hours.
- Housing: Individual housing in stainless steel wire mesh, battery type cages (dimensions: 16 crn x 27 cm and a height of 20-23 cm).
- Diet (e.g. ad libitum): Free access to a standard commercial avian diet, (= basal diet) Special Diet Services, Witham, Essex, United Kingdom.
- Water (e.g. ad libitum): Free access to tap water.
- Acclimation period: 15 days under laboratory conditions.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 15.5-23
- Humidity (%): 56-68
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 8/16
IN-LIFE DATES: 16 December 1998 - 30 December 1998
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: Concentration of test substance in vehicle was varied to allow constant dosage volume in terms of ml/kg body weight.
- Amount of vehicle (if gavage): doding volume was maximally 5 ml/kg
- Justification for choice of vehicle: The vehicle was selected based on trial formulations performed at NOTOX and/or test substance data supplied by the sponsor.
- Lot/batch no. (if required): not applicable
- Purity: not applicable
MAXIMUM DOSE VOLUME APPLIED: 5 ml/kg
DOSAGE PREPARATION (if unusual): The test formulation was homogenised to a visually acceptable level by stirring. The test formulation was dosed within 2 hours after preparation.
- Doses:
- 191, 343, 617, 1111 and 2000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
Mortality:
On day of dosing: continuously during the first 1 to 2 hours after dosing, and subsequently every 2 hours (at least three times). During remainder of study period: twice a day.
Clinical signs:
On day of dosing: continuously during the first 1 to 2 hours after dosing, and subsequently every 2 hours (at least three times). Durjng remainder of study period: once a day. All signs of reaction to treatment were recorded and graded according to fixed scales (specified in the report). The time of onset and duration was recorded. Mortality and clinical signs were recorded in the computer.
Body weights:
Day before dosing (when capsules are used), day 1 (day of dosing), day 8, day 15 and at death (from day 2 onwards).
- Necropsy of survivors performed: yes, necropsy and descriptions of all internal macroscopic abnormalities were recorded. Gross necropsies included general inspection of the gastrointestinal tract, liver , kidney, heart and spleen.
- Other examinations performed:
Food consumption:
Food consumption was monitored from days 1-4, 4-8, 8-11 and 11-15. - Statistics:
- The LD50 value, the associated 95% confidence limits and the slope of the dose-response line were calculated in terms of the quantity of active ingredient or total formulation (as appropriate) per unit body weight. The LD50 value was calculated using the Maximum Likelihood method (Finney, D.G.,
Probit Analyses, 3rd Edition, London, Cambridge University Press, 1971). Group means were calculated for continuous data.
The following statistical methods were used to analyse body weights and food consumption:
- Since variables were assumed to follow a normal distribution, the Dunnett test (many-to-one t-test) based on a pooled variance estimate was applied for the comparison of the treated groups and the control groups for each sex.
Test statistics were calculated on the basis of exact values for means and pooled variances. Individual values, means and standard deviations may have been rounded off before printing. Therefore, for example, two groups may display the same printed means for a given parameter, yet display different test statistics values.
The test is invalid if more than 10% o f the birds in the control group die. No compound-related, observable toxic effects should be recorded in the lowest test concentration.
Results and discussion
- Preliminary study:
- Treatment:
The test substance was administered as a single dose by oral gavage using water {milli-U) as a vehicle at dose concentrations of 20, 200 and 2000 mg/kg body weight. The dose volume was 5 ml/kg body weight. One male and one female were used per dose group.
Observations:
Birds were checked for mortality, clinical signs and body weights in a similar way as described for the main study with the exception that the study
continued for 8 days.
Results:
The two birds of the 2000 mg/kg group died within one hour after dosing. Clinical signs observed were clonic spasms, tremors of the head, ventrolateral recumbency, hunched posture, uncoordinated movements, breathing abnormalities, fluid faeces and ptosis in the highest dosage group prior to death. Fluid faeces was noted at 200 mg/kg and 20 mg/kg. No effects on body weights were found in the surviving birds.
Conclusions:
Based on these results it was decjded to conduct a full study with dose levels selected to be 0, 191, 343, 617, 1111 and 2000 mg/kg body weight.
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 508 mg/kg bw
- 95% CL:
- 349 - 693
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 508 mg/kg bw
- 95% CL:
- 285 - 790
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 508 mg/kg bw
- 95% CL:
- 285 - 790
- Mortality:
- None of the animals died in group 1-3 (0, 191, 343 mg/kg bw) and all animals died in groups 4-6 (617, 1111, 2000 mg/kg bw) .All deaths of group 4 (617 mg/kg) were recorded within 5 hours after dosing except for one male, which was found dead on day 2 after treatment. Four deaths of group 5 (1111 mg/kg) were observed within 3 hours, the remaining deaths of this group and all of group 6 (2000 mg/kg) were recorded within 1 hour after dosing.
- Clinical signs:
- other: The following clinical findings were noted: 0 mg/kg: Fluid faeces until 3 hours after dosing in all birds. 191 mg/kg: Fluid faeces until 5 hours after dosing in all birds. 343 mg/kg : Fluid faeces until day 2 in all birds. Uncoordinated movements, letharg
- Gross pathology:
- 0 mg/kg: No findings noted.
191 mg/kg: No findings noted.
343 mg/kg: No findings noted.
617 mg/kg: Haemorrhages in the intestines, swollen andlor dark red liver, a dark red spleen and a detached cuticle of the
stomach were noted among the birds.
1111 mg/kg: Haemorrhages in the intestines, swollen andlor dark red liver, and detached cuticles of the stomach were noted
among the birds.
2000 mg/kg: Haemorrhages in the intestines, swollen andlor dark red liver and detached cuticles of the stomach were noted among
the birds.
The findings in the dosage groups of 617 mg/kg and above were considered to be adverse effects of treatment with the test substance. - Other findings:
- Mean food intake was statistically significantly decreased far both sexes in the 343 mg/kg group after 4 days of treatment when comparing with the control group. This was considered to be an adverse effect of administration of ammonium thiocyanate. Due to death of the birds, non-relevant statistical significant differences were obtained for the higher dose groups. No effect on mean food consumption was seen at 191 mg/kg.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Remarks:
- Migrated information
- Conclusions:
- Under the conditions of this study, the oral LD values of ammoniumthiocyanate in Japanese quail for the sexes combined was established as 508
mg/kg body weight with 95% confidence limits being 349 - 693 mg/kg and the slope of the dose-response curve 3.11. For the individual sexes the LD50 values were also established to be 508 mg/kg body weight with the 95% confidence intervals being 285 - 790 mg/kg and the slope being 2.45. The no observed effect level (NOEL) in this study was 191 mg/kg body weight. - Executive summary:
This study was performed to assess the acute thiocyanate to the Japanese quail when administered as a single oral dose. The study carried out was based on the OECD Guideline No. 401 and on the EPA FIFRA Ref. No. 71-1, EPA TSCA Par. 797.2175, EPA OPPTS Par. 850.2100 Guidelines, with the exception that the study was performed with Japanese quails.
Treatment
Ammonium thiocyanate was administered by oral gavage, using water (Milli-U) as a vehicle, to five birds of each sex at 191, 343, 617, 1111 or 2000 mg/kg body weight. A control group of five birds of each sex was dosed with vehicle (5 ml/kg). Birds were observed at periodic intervals on the day of dosing and daily thereafter; body weight was determined at start of the study (day 1), day 8 and day 15; food consumption was measured from days 1-4, 4-8, 8-11 and 11-15. Macroscopic post-mortem examination was performed at termination (day 15).
Results
0 mg/kg: 1) Fluid faeces.
191 mg/kg: 1) Fluid faeces.
343 mg/kg: 1) Fluid faeces, uncoordinated movements, lethargy, clonic spasms, abnormal posture of the head, ventro-lateral recumbency, hunched posture, breathing abnormalities or ptosis were seen among the birds.
2) Reduced food consumption during 4 days after dosing.
1111 mg/kg 1) All birds died within 5 hours after dosing, except for one male which was found dead on day 2. 2 )Clinical signs noted were fluid or red faeces, uncoordinated movements, lethargy, clonic spasms, abnormal posture of the head, tremors of the head or wing, ventro-lateral recumbency, hunched posture, breathing abnormalities and ptosis. 3) Examination post-mortem revealed haemorrhages in the intestines, swollen andlor dark red liver, dark red spleen and a detached cuticle of the stomach.
2000 mg/kg
1) All birds died within 1 hour post-dosing.
2) Clinical signs noted were fluid faeces, uncoordinated movements, lethargy, clonit spasms, tremors or abnormal posture of the head, ventro-lateral recumbency, hunched posture, breathing abnormalities and ptosis.
3) Examination post-mortem revealed haemorrhages in the intestines, swollen andlor dark red liver and detached cuticles of the stomach.
Conclusion
Under the conditions of this study, the oral LD50 values of ammonium thiocyanate in Japanese quail was established as 508 mg/kg body weight with 95% confidence limits being 349 - 693 mg/kg and the slope of the dose-response curve 3.11. For the individual sexes the LD50 values were also established to be 508 mg/kg body weight with the 95% confidence intervals being 285 - 790 mg/kg and the slope being 2.45. The no observed effect level (NOEL) in this study was 191 mg/kg body weight.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)
Although ECHA is providing a lot of online material in your language, part of this page is only in English. More about ECHA’s multilingual practice.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
the-echa-website-uses-cookies
find-out-more-on how-we-use-cookies