Oxybis(methyl-2,1-ethanediyl) diacrylate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1984

Report date:

1982

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

abraded skin

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Oxybis(methyl-2,1-ethanediyl) diacrylate

EC Number:

260-754-3

EC Name:

Oxybis(methyl-2,1-ethanediyl) diacrylate

Cas Number:

57472-68-1

Molecular formula:

C12H18O5

IUPAC Name:

oxydipropane-1,2-diyl bisacrylate

Details on test material:

- Name of test material (as cited in study report): A209415
- Physical state: liquid
- Analytical purity: no data
- Storage condition of test material: room temperature away from light

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Clerco Research Farms
- Age at study initiation: young adults
- Weight at study initiation: 2.75 to 3.10 kg
- Housing: individually housed in stainless steel wire-bottomed cages
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: 26 days


ENVIRONMENTAL CONDITIONS
no data

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: 240 cm2
- % coverage: 10 %
- Type of wrap if used: the test site was occluded with a layer of 4-ply gauze, two single layers thick. The trunk of the rabbit was wrapped with rubber
latex dental dam and the dental dam, taped at the edges with 1 inch Micropore, tape to form an airtight occlusive wrap.


REMOVAL OF TEST SUBSTANCE
after 24 hours the residual test material was wiped off


TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1.88 ml/kg
- Constant volume or concentration used: yes

Duration of exposure:

24 hours

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

5 male and 5 female animals

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: frequently on the day of dosing and twice daily thereafter, weighing on the day before the study, day 0, day 6 and day 13
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Statistics:

not applicable

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

no deaths

Clinical signs:

other: signs of systemic toxicity were observed on day 1 gradually decreasing in severity and incidence to day 6, when they were no longer present. These signs consisted of the following: eyes appear bluish, ears cool to touch, increased rate of respiration, hun

Gross pathology:

lungs spotted dark red in color in 1/5 female animals, lungs dark red in color in 4/5 male and 1/5 female animals. No significant other pathological
findings.

Other findings:

Evaluation of local skin reactions revealed: erythema (10/10), edema (10/10), hemorrhage (9/10), eschar formation (9/10), fissuring (7/10), scattered raw areas (3/10), scaling (4/10), coriaceous (6/10), abraded lines well defined (2/10) and compound residue present (10/10)