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Diss Factsheets
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EC number: 224-073-5 | CAS number: 4193-55-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From May 12 to15, 1987
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Test conducted according to internationally accepted testing guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Version / remarks:
- adopted May 12, 1981
- GLP compliance:
- no
Test material
- Reference substance name:
- Disodium 4,4'-bis[6-anilino-[4-[bis(2-hydroxyethyl)amino]-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate
- EC Number:
- 224-073-5
- EC Name:
- Disodium 4,4'-bis[6-anilino-[4-[bis(2-hydroxyethyl)amino]-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate
- Cas Number:
- 4193-55-9
- Molecular formula:
- C40H44N12O10S2.2Na
- IUPAC Name:
- disodium 2-[(1E)-2-[4-({4-[bis(2-hydroxyethyl)amino]-6-(phenylamino)-1,3,5-triazin-2-yl}amino)-2-sulfonatophenyl]ethenyl]-5-({4-[bis(2-hydroxyethyl)amino]-6-[(cyclohexa-1,3-dien-1-yl)amino]-1,3,5-triazin-2-yl}amino)benzene-1-sulfonate
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Kleintierfarm Madoerin AG, CH-4414 Fuellinsdorf.
- Age at study initiation: approx. 12-14 weeks old.
- Weight at study initiation: between 2190 t o 2470 g.
- Housing: individually housed in metal cages.
- Diet: ad libitum, standard rabbit pellet NAFAG, N. 814 Gossau. All batches of the diet are assayed for nutritive ingredients and contaminant level by the manufacturer.
- Water: ad libitum. The quality of the drinking water was according to the specification of the "Schweizerisches Lebensmittelbuch" (Edition 1972).
- Acclimation period: 5 days.
ENVIRONMENTAL CONDITIONS
- Temperature: constant room temperature of 20 ± 3 °C
- Humidity: relative humidity of 30-70 %
- Photoperiod: 12 hrs light cycle day.
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- shaved
- Vehicle:
- water
- Controls:
- yes, concurrent vehicle
- Amount / concentration applied:
- A gauze patch (20 cm2) bearing 0.5 g of the test substance was applied to the flank.
A control gauze patch was applied to the contra-lateral flank. Both gauze patches were moistened before application with distilled water. - Duration of treatment / exposure:
- 4 hours
- Observation period:
- The skin reactions were evaluated 1, 24, 48, and 72 hours after removing the gauze patches.
- Number of animals:
- 3 males
- Details on study design:
- TEST SITE
- Area of exposure: an area of at least 6 cm2 was shaved on both flanks of the animals approximately 24 hours before treatment.
- Type of wrap if used: the patches were loosely covered with an aluminium foil (36 cm2 ) and held in place for 4 hours by an adhesive tape (Isoplast, Isoplast AG, CH-5200 Brugg).
OBSERVATION
The animals were checked daily for systemic symptoms and mortality.
SCORING SYSTEM
According to the OECD scoring system.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- ca. 0
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritation parameter:
- erythema score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- ca. 0
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritation parameter:
- erythema score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- ca. 0
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- ca. 0
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- ca. 0
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritation parameter:
- edema score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- ca. 0
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritant / corrosive response data:
- Because no reactions were observed at 24 hours to 7 2 hours after removing the bandages, the test was ended after the 72 hours evaluation.
It can be concluded that test item has no primary skin irritant potency in albino rabbits.
Any other information on results incl. tables
Evaluation of skin reaction
Animal no. | Erythema | Oedema | ||||
25/M | 26/M | 27/M | 25/M | 26/M | 27/M | |
CF/TF | CF/TF | CF/TF | CF/TF | CF/TF | CF/TF | |
After 1 hr | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 |
After 24 hrs | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 |
After 48 hrs | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 |
After 72 hrs | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 |
mean 24-72 hrs | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 |
CF = control flank; TF = test flank
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information according to the CLP Regulation Criteria used for interpretation of results: EU
- Conclusions:
- Not irritating.
- Executive summary:
Method
The skin irritation potential was assessed according to the OECD guideline No. 404, adopted May 12, 1981, by the OECD council.
Results
Under the experimental conditions employed test item did not induce skin irritation when applied to the clipped albino rabbit skin. Because no reactions were observed at 24 hours to 72 hours after removing the bandages, the test was ended after the 72 hours evaluation.
Conclusion
It can be concluded that test item has no primary skin irritant potency in albino rabbits.
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