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EC number: 931-299-4
CAS number: 68390-94-3
Table 1. Validity criteria for OECD 218.
Criterion from the guideline
Validity criterion fulfilled
The emergence in the control must be at least 70% at the end of the test.
C. riparius and C. yoshimatsui emergence to adults from control vessels should occur between 12 and 23 d after their insertion into the vessels. For C. tentans, a period of 20 to 65 d is necessary.
> day 16
At the end of the test, pH and the dissolved oxygen concentration should be measured in each vessel. The oxygen concentration should be at least 60% of the air saturation value (ASV) at the temperature used and the pH of the overlying water should be in the 6-9 range in all test vessels.
The water temperature should not differ by more than ± 1.0 °C.
22 ± 1 °C
The substance is not toxic to sediment organisms (NOEC(28 d) ≥ 1000 mg/kg).
The toxicity of the substance to sediment-dwelling larvae of
Chironomus riparius was tested according to OECD 218 (GLP)).
Following a preliminary range-finding test 80 larvae of Chironomus
riparius were exposed in groups of 20 (four replicates of 20 larvae per
concentration) to formulated sediment spiked with test item
concentrations of 63, 125, 250, 500 and 1000 mg/kg for a period of 28
days. The numbers of emerged adult midges were recorded daily. A another
replicate with 20 larvae of each test group was prepared and sacrificed
on Day 10 of the exposure period to determine the 10-Day larval survival
and growth data. Inspection of the data showed no significant
differences in larval survival and growth, in terms of mean larval dry
weight, between the control and each test group after 10 days.
The toxicity of the test item to the sediment-dwelling larvae of
Chironomus riparius gave a 28-Day EC50 (emergence) of > 1000 mg/kg. The
NOEC (28 d) was ≥ 1000 mg/kg. The EC50 (development rate) based on
nominal test concentrations was > 1000 mg/kg.
Based on the available study results, the substance is not
considered toxic to sediment organisms.
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