2,4,6-trinitrotoluene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

GLP compliance:

yes (incl. QA statement)

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

2,4,6-trinitrotoluene

EC Number:

204-289-6

EC Name:

2,4,6-trinitrotoluene

Cas Number:

118-96-7

Molecular formula:

C7H5N3O6

IUPAC Name:

2-methyl-1,3,5-trinitrobenzene

Details on test material:

TNT 99.95% purity.
The test item, 2,4,6-Trinitrotoluene was administered following sieving and grinding. The test item was sieved and ground by mortar and pestle (to allow a viable aerosol to be produced), and stored at ambient conditions until use.

Test animals

Species:

rat

Strain:

Crj: CD(SD)

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

nose only

Vehicle:

air

Analytical verification of test atmosphere concentrations:

no

Remarks:

The carrier air into the exposure chamber and the extraction from it was monitored visually using calibrated flow meters and recorded at approximately 30 minute intervals throughout the exposure period.

Duration of exposure:

4 h

Concentrations:

The target concentration of 1.0 mg/L was selected based on the known oral toxicity (LD50) of 2,4,6-Trinitrotoluene.

No. of animals per sex per dose:

5 males, 5 females

Control animals:

no

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 1.01 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Mortality:

There was no mortality observed during the study period.

Clinical signs:

other: Slow respiration, salivation and red liquid apparent in restraint tube were noted in all animals during the 4 hour exposure. Subdued behaviour, laboured respiration, rolling gait, piloerection, unkempt coat, and red staining, abdomen were noted post expos

Body weight:

All animals’ body weight profile was satisfactory throughout the study period

Gross pathology:

No necropsy findings were noted in any animal

Any other information on results incl. tables

The following parameters and end points were evaluated in this study: clinical signs, body weights, body weight changes, and gross necropsy findings.

In conclusion, following a single 4 hour inhalation exposure to 2,4,6-Trinitrotoluene via snout only method no mortality or overt toxicity was observed during exposure or post exposure observation period. The LC50 is considered to be in excess of 1.01 mg/L.

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Remarks:

Based on harmonized classification.

Conclusions:

In conclusion, following a single 4 hour inhalation exposure to 2,4,6-Trinitrotoluene via snout only method no mortality or overt toxicity was observed during exposure or post exposure observation period. The LC50 is considered to be in excess of 1.01 mg/L.

Executive summary:

Based on study results the substance meets category 4 criteria for dust/mist. However, the result is a boardline with harmonized classification and worst case scenario is applied.