1,6,10-Dodecatriene, 7,11-dimethyl-3-methylene-, (6E)-, hydrogenated

Water solubility

Administrative data

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experimental Starting Date: 09 June 2014 Experimental Completion Date: 22 August 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results. The study report was conclusive, done to a valid guideline and the study was conducted under GLP conditions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

GLP compliance:

yes

Type of method:

flask method

Remarks:

The determination was carried out using a flask method, designed to be compatible with Method A6 Water Solubility of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 105 of the OECD Guidelines for Testing of Chemicals, 27 July 1995.

Test material

Test material information

Constituent 1

Reference substance name:

1.25H Farnesene

IUPAC Name:

1.25H Farnesene

Test material form:

other: Clear colorless liquid

Details on test material:

Identification: 1.25H Farnesene
Appearance/Physical State: Clear colorless liquid
Lot: RV-752-153-2
Purity: 98.92%
Expiry Date: 01 March 2015
Storage Conditions: Approximately 4 °C, in the dark

Results and discussion

Water solubility

Water solubility:

<= 0 g/L

Temp.:

20 °C

Any other information on results incl. tables

Results

Preliminary Test:

The preliminary estimate of water solubility was 3.16 x 10^-6 g/L at 20 °C.

Main Test:

Please see Attachment 1 for Typical Chromatography.

The mean peak areas relating to the standard and sample solutions are shown in the following tables:

Table 3.2 – Analysis of samples 1 and 3

 

Solution	Mean PeakArea
Standard at 0.104 mg/L	4.424 x 10^4
Standard at 0.112 mg/L	4.858 x 10^4
Sample 1A	5.327 x 10^4
Sample 1B	5.836 x 10^4
Sample 3A	7.467 x 10^4
Sample 3B	7.466 x 10^4
Standard at 0.104 mg/L	5.531 x 10^4
Standard at 0.112 mg/L	5.666 x 10^4

Table 3.3 – Analysis of sample 2

 

Solution	Mean Peak Area
Standard at 0.105 mg/L	1.041 x 10^5
Standard at 0.104 mg/L	9.494 x 10^4
Sample 2A	1.136 x 10^5
Sample 2B	1.185 x 10^5
Standard at 0.105 mg/L	1.030 x 10^5
Standard at 0.104 mg/L	9.518 x 10^4

 

The concentration (g/L) of test item in the sample solutions is shown in the following table:

Table 3.4

 

Sample Number	Time Shaken at ~ 30 ºC (h)	Time Equilibrated at 20 ºC (h)	Concentration (g/L)	Solution pH
1	24	24	2.94 x 10^-6	5.66
2	48	24	3.05 x 10^-6	5.78
3	72	24	3.94 x 10^-6	5.81

 

The sample concentrations (g/L) were then corrected by the recovery of analysis result (86.4%, see the validation section) for the sample procedure at a nominal concentration level of 2.5 x 10^-6g/L.

 

Table 3.5

 

Sample Number	Concentration Corrected by Recovery (g/L)
1	3.41 x 10^-6
2	3.53 x 10^-6
3	4.56 x 10^-6

 

Limit concentration    :          ≤4.56 x 10^-6 g/L at 20.0 ± 0.5 ºC

Validation

The linearity of the detector response with respect to concentration was assessed over the nominal concentration range of 5 x 10^-2 to 0.2 mg/L. This was satisfactory with a correlation coefficient of 1.000 being obtained.

 

Recovery of analysis for the sample procedure was assessed and proved adequate for the test. The recovery data is shown in the following table:

 

Table 3.6

 

Nominal Concentration (g/L)	Recovery Range (%)	Mean Recovery (%)
1.0 x 10^-5	81.1 to 87.3	84.0
5.0 x 10^-6	87.4 to 97.0	90.8
2.5 x 10^-6	79.8 to 93.8	86.4

 

 

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): insoluble (< 0.1 mg/L)
Please see the Executive Summary below for the Conclusion.

Executive summary:

The determination was carried out using a flask method, designed to be compatible with Method A6 Water Solubility of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 105 of the OECD Guidelines for Testing of Chemicals, 27 July 1995.

 

The test system consists of purified water with a resistivity of greater than 15MΩ.

        

Conclusion

The water solubility of the test item has been determined to be less than or equal to 4.56 x 10^-6 g/L of solution at 20.0 ± 0.5 °C.