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EC number: 220-342-6 | CAS number: 2726-73-0
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The 96-h LL50 value is >100 mg/L (loading rate) in rainbow trout (O. mykiss).
Key value for chemical safety assessment
Additional information
The short-term toxicity to fish was determined in a limit test according to OECD guideline No 203 and in compliance with GLP criteria (Institute of Industrial Organic Chemistry Branch Pszczyna, 2015).
In this study groups of 7 rainbow trout (O. mykiss) were exposed to test substance loading rates of 0 (control) and 100 mg/L for 96 hours under static conditions. The filtrate of the 100 mg/L loading rate was analytically verified based on total organic carbon (TOC) content at t=0, 24, 48, 72 and 96 hours after exposure. This analytical approach did not account for any degradation or metabolisation effects which may have occurred during exposure. The analytical concentrations for the filtrate of the 100 mg/L loading rate were all below the LoQ for TOC of 1.0 mg C/L. In the control TOC values ranged from 1.32 to 1.76 mg C/L. Fish were observed for survival and changes in behavior, respiration and pigmentation after 3, 6, 24, 48, 72 and 96 h of exposure.
At the end of the test no mortality of fish and no symptoms of intoxication were observed in the control and in the filtrate of a loading of 100 mg/L. Based on these findings the 96 -h LL50 value was determined at >100 mg/L.
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