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EC number: 220-780-8 | CAS number: 2897-60-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-09-02 to 1999-11-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- [3-(2,3-epoxypropoxy)propyl]diethoxymethylsilane
- EC Number:
- 220-780-8
- EC Name:
- [3-(2,3-epoxypropoxy)propyl]diethoxymethylsilane
- Cas Number:
- 2897-60-1
- Molecular formula:
- C11H24O4Si
- IUPAC Name:
- diethoxy(methyl){3-[(oxiran-2-yl)methoxy]propyl}silane
- Reference substance name:
- [3-(2,3-epoxypropoxy)propyl]diethoxy(methyl)silane
- IUPAC Name:
- [3-(2,3-epoxypropoxy)propyl]diethoxy(methyl)silane
Constituent 1
Constituent 2
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor induced rat liver S9
- Test concentrations with justification for top dose:
- 100, 333, 1000, 3333, 5000 μg/ plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: Solubility properties and relative non-toxicity to bacteria
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- All salmonella strains + WP2 uvrA (with activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- TA 98 (without activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- TA 100, TA 1535 (without activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- TA 1537 (without activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Remarks:
- WP2 uvrA (without activation)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar ; preincubation
ACTIVATION: The S9 mix contained 10 % S9, 5 mM glucose-6-phosphate, 4 mM β-nicotinamide-adenine dinucleotide phosphate, 8 mM MgCl2 and 33 mM KCl in 100 mM phosphate buffer at pH 7.4.
DURATION
- Preincubation period: 60 minutes
- Expression time (cells in growth medium): 48 to 72 hours
NUMBER OF REPLICATIONS: 3 plates for each test concentration
DETERMINATION OF CYTOTOXICITY
- Method: Background lawn analysis
- Evaluation criteria:
- A result is positive if the number of revertants is significantly increased compared with the solvent control to at least 2-fold of the solvent control for TA 98, TA 100 and WP2 uvrA and 3-fold of the solvent control for TA 1535 and TA 1537.
Cytotoxicity is defined as a reduction in the number of colonies by >50% compared with the solvent control and/or a sparse background lawn.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- reduced background lawn3333 - 5000 μg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Remarks:
- 2.1 - 3.1-fold increase in revertants
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- reduced background lawn 5000 μg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Remarks:
- 4.1 - 9.5-fold increase in revertants
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- reduced background lawn 3333 - 5000 μg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Remarks:
- 13.1 - 17-fold increase in revertants
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Remarks:
- 10.4 - 21.8-fold increase in revertants
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- reduced background lawn 3333 - 5000 μg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- reduced background lawn 5000 μg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- reduced background lawn 3333 - 5000 μg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Remarks:
- 3.2 - 4.4-fold increase in revertants
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Remarks:
- 4.7 - 5.2-fold increase in revertants
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
Any other information on results incl. tables
Table 2: Preliminary toxicity assay for all strains with and without metabolic activation
|
TA98 |
TA100 |
TA1535 |
||||||
Conc. |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
0* |
14 |
26 |
No |
171 |
160 |
No |
23 |
15 |
No |
6.7 |
15 |
NC** |
No |
183 |
147 |
No |
20 |
17 |
No |
10 |
14 |
12 |
No |
167 |
187 |
No |
25 |
19 |
No |
33 |
12 |
26 |
No |
173 |
204 |
No |
24 |
14 |
No |
67 |
22 |
24 |
No |
205 |
175 |
No |
22 |
20 |
No |
100 |
16 |
19 |
No |
209 |
190 |
No |
26 |
22 |
No |
333 |
12 |
18 |
No |
325 |
193 |
No |
36 |
22 |
No |
667 |
12 |
22 |
No |
374 |
186 |
No |
40 |
22 |
No |
1000 |
18 |
22 |
No |
614 |
188 |
No |
50 |
27 |
No |
3333 |
17 |
18 |
No |
862 |
324 |
No |
237 |
37 |
No |
5000 |
17 |
20 |
No |
928 |
462 |
No |
418 |
97 |
No |
*solvent control with DMSO
**NC = No count due to procedural error in which plate did not receive an aliquot of tester strain
Table 2: Preliminary toxicity assay for all strains with and without metabolic activation
|
TA1537 |
WP2 uvrA |
||||
Conc. |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
0* |
13 |
13 |
No |
22 |
17 |
No |
6.7 |
12 |
15 |
No |
17 |
11 |
No |
10 |
12 |
19 |
No |
14 |
13 |
No |
33 |
11 |
14 |
No |
14 |
11 |
No |
67 |
12 |
13 |
No |
13 |
17 |
No |
100 |
10 |
6 |
No |
18 |
13 |
No |
333 |
15 |
15 |
No |
21 |
22 |
No |
667 |
11 |
12 |
No |
14 |
17 |
No |
1000 |
13 |
10 |
No |
27 |
23 |
No |
3333 |
24 |
10 |
No |
40 |
24 |
No |
5000 |
20 |
12 |
No |
38 |
31 |
No |
*solvent control with DMSO
Table 3: Experiment 1 Preincubation Number of revertants per plate (mean of 3 plates)
|
TA98 |
TA100 |
TA1535 |
||||||
Conc. |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
0* |
32 |
36 |
No |
100 |
118 |
No |
14 |
10 |
No |
100 |
26 |
32 |
No |
149 |
110 |
No |
26 |
12 |
No |
333 |
24 |
33 |
No |
211 |
108 |
No |
54 |
22 |
No |
1000 |
22 |
28 |
No |
453 |
127 |
No |
87 |
28 |
No |
3333 |
27** |
35 |
Yes |
705** |
249 |
Yes |
145** |
104 |
Yes |
5000 |
28** |
26 |
Yes |
948** |
363 |
Yes |
120** |
170 |
Yes |
Positive control |
640 |
544 |
No |
410 |
522 |
No |
248 |
78 |
No |
*solvent control with DMSO
**moderately reduced background lawn
***extremely reduced background lawn
Table 3: Experiment 1 Preincubation Number of revertants per plate (mean of 3 plates)
|
TA1537 |
WP2 uvrA |
||||
Conc. |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
0* |
13 |
19 |
No |
12 |
13 |
No |
100 |
13 |
16 |
No |
10 |
10 |
No |
333 |
13 |
22 |
No |
15 |
15 |
No |
1000 |
16 |
9 |
No |
26 |
18 |
No |
3333 |
9** |
18 |
No |
62 |
22 |
No |
5000 |
6*** |
20 |
Yes |
58 |
42 |
No |
Positive control |
829 |
89 |
No |
188 |
54 |
No |
*solvent control with DMSO
**moderately reduced background lawn
***extremely reduced background lawn
Table 4: Experiment 2 Preincubation Number of revertants per plate (mean of 3 plates)
|
TA98 |
TA100 |
TA1535 |
||||||
Conc. |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
0* |
12 |
18 |
No |
125 |
138 |
No |
9 |
9 |
No |
33 |
11 |
19 |
No |
135 |
190 |
No |
10 |
6 |
No |
100 |
13 |
18 |
No |
139 |
121 |
No |
13 |
12 |
No |
333 |
12 |
17 |
No |
196 |
121 |
No |
32 |
14 |
No |
1000 |
11 |
15 |
No |
350 |
145 |
No |
45 |
19 |
No |
3333 |
14** |
13 |
Yes |
383** |
270 |
Yes |
141 |
67 |
No |
5000 |
6*** |
18** |
Yes |
518*** |
288** |
Yes |
196** |
118 |
Yes |
Positive control |
527 |
597 |
No |
378 |
622 |
No |
165 |
32 |
No |
*solvent control with DMSO
**moderately reduced background lawn
***extremely reduced background lawn
Table 4: Experiment 2 Preincubation Number of revertants per plate (mean of 3 plates)
|
TA1537 |
WP2 uvrA |
||||
Conc. |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
0* |
6 |
6 |
No |
11 |
13 |
No |
33 |
4 |
8 |
No |
11 |
9 |
No |
100 |
5 |
5 |
No |
10 |
11 |
No |
333 |
4 |
4 |
No |
9 |
8 |
No |
1000 |
2 |
5 |
No |
16 |
19 |
No |
3333 |
4** |
6 |
Yes |
50 |
33 |
No |
5000 |
5*** |
6** |
Yes |
52 |
57 |
No |
Positive control |
396 |
59 |
No |
207 |
134 |
No |
*solvent control with DMSO
**moderately reduced background lawn
***extremely reduced background lawn
Applicant's summary and conclusion
- Conclusions:
- Under test conditions, there was a positive, dose related increase in the number of revertant colonies in Salmonella typhimurium TA 100, TA 1535 and E. coli WP2 uvrA, with and without metabolic activation. The test substance is mutagenic in these tester strains.
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