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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study was performed according to German national test guidelines. The solvent was not constant within all test concentrations.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DIN 38412 L9
GLP compliance:
no

Test material

Constituent 1
Reference substance name:
Citral
EC Number:
226-394-6
EC Name:
Citral
Cas Number:
5392-40-5
IUPAC Name:
3,7-dimethylocta-2,6-dienal
Details on test material:
- Name of test material (as cited in study report): Citral N (3,7-Dimethyl-2,6-octadien-l-al)
- Lot/batch No.: B2490 from 11.04.88
Purity: No data

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Cremophor

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Nominal and measured concentrations:
Nominal test concentrations:
0.5, 1.0, 2.5, 5.0, 10.0, 25.0, control, solvent control

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
103.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Any other information on results incl. tables

EC values after 72 hours (in mg/l):

EbC10: 4.9 (95%-confidence intervals: 1.8 - 13.3)
EbC50: 16.1 (95%-confidence intervals: 3.7 - 71.0)

ErC10: 3.0 (95%-confidence intervals: 2.0 - 4.6)
ErC50: 103.8 (95%-confidence intervals: 33.8 - 318.9)


EC values after 96 hours (in mg/l):

EbC10: 1.9 (95%-confidence intervals: 1.4 - 2.4)
EbC50: 18.9 (95%-confidence intervals: 13.0 - 27.4)

ErC10: 8.9 (95%-confidence intervals: 3.2 - 25.1)
ErC50: 103.8 (95%-confidence intervals: 4.9 - 117.4)

Applicant's summary and conclusion