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EC number: 700-678-4 | CAS number: 1076-12-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 December 2013 - 25 January 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Nominal concentrations 0.0811, 0.178, 0.393, 0.864, and 1.90 mg/L.
- Sampling method: Samples were diluted with acetone prior to quantitation by gas chromatography with mass spectrometric detection (GC-MS).
- Sample storage conditions before analysis: Not applicable - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls: A solvent control containing 100µL/L THF in dilution water was prepared
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tetrahydrofuran
- Concentration of vehicle in test medium (stock solution and final test solution): 100µL/L (0.1 mL in 1000 mL)
- Evidence of undissolved material (e.g. precipitate, surface film, etc): None recorded
Note that test vessels were filled completely without any headspace, adn sealed using teflon lids and metal caps, due the issues caused by the volatility of the test substance. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: Straus
- Age at study initiation: Less than 24 hours old
ACCLIMATION (Culture conditions prior to test)
- Type and amount of food: Pseudokirchneriella subcapitata
- Feeding frequency: 0.1 to 0.2 mg carbon per daphnid per day
- Health during acclimation (any mortality observed): Not recorded. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 254 mg/L (first batch) and 268 mg/L (second batch) as CaCO3.
- Test temperature:
- 19.0 to 19.7°C
- pH:
- 7.93 to 8.16
- Dissolved oxygen:
- 9.01 to 9.57 mg/L
- Nominal and measured concentrations:
- Nominal: 0.0811, 0.178, 0.393, 0.864, and 1.90 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Wheaton vials
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Completely filled (c.a. 120 mL) allowing no headspace and sealed with teflon lids with metal caps
- Aeration: None
- Renewal rate of test solution (frequency/flow rate): Test medium was renewed every 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate: 24 mL medium per organism.
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light: 8 hours dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilisation, recorded at 24 and 48 hours from study initiation. Daphnia were considered to be immobile if they were unable to swim within approximately 15 seconds following gentle agitation of the test vessel.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Range finding study
- Test concentrations: 0.019, 0.19, 1.90 mg/L. 100% immobilistation seen at 1.90 mg/L level, no immobilisation seen at other (0.019 and 0.19 mg/L) levels.
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.158 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL 0.129 - 0.170 mg/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.055 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.129 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.201 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL 0.186 - 0.245 mg/L
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.129 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.245 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: No Daphnia in the control or solvent control groups were immobile after 48 hours.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: There was no evidence of undissolved material. - Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of TCD to Daphnia magna was assessed, in semi-static exposure conditions and in a sealed test system, over 48 hours, with renewal of media at 24 hours.
The 48-hour EC50 of TCD for the immobilisation of Daphnia magna was 0.158 mg/L (95% confidence limits of 0.129 and 0.17 mg/L) based on measured concentrations of TCD.
The 48 hour ‘no-observed effect concentration’ NOEC of TCD with Daphnia magna was 0.0553 mg/L (measured concentration) TCD.
Reference
Description of key information
48-Hour EC50 to Daphnia Magna = 0.158 mg/L. NOEC = 0.0553 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.158 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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